Mass Spectrometry in Biology & Medicine

1. Site-specific Characterization of the N-linked Glycans of Murine PrPSc Using Advanced Methods of Electrospray Mass Spectrometry

   • Elaine Stimson, James Hope, Angela Chong, Alma L. Burlingame

   Pages 383-402

2. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of N-Linked Carbohydrates and Related Compounds

   • David J. Harvey, Bernhard Küster, Susan F. Wheeler, Ann P. Hunter, Robert. H. Bateman, Raymond A. Dwek

   Pages 403-437

3. Electrophoretic and Mass Spectrometric Strategies for the Identification of Lipopolysaccharides and Immunodeterminants in Pathogenic Strains of Haemophilus influenzae; Application to Clinical Isolates

   • P. Thibault, J. Li, A. Martin, J. C. Richards, D. W. Hood, E. R. Moxon

   Pages 439-462

4. Mycobacterial Lipoglycans: Structure and Roles in Mycobacterial Immunity

   • J. Nigou, B. Monsarrat, M. Gilleron, T. Brando, R. Albigot, G. Puzo

   Pages 463-480

5. The Impact of Drug Metabolism in Contemporary Drug Discovery: New Opportunities and Challenges for Mass Spectrometry

   • Thomas A. Baillie, Paul G. Pearson

   Pages 481-496

6. Electrospray Mass Spectrometric Analysis of Lipid Mediators Derived from Arachidonic Containing Membrane Phospholipids

   • Robert C. Murphy, Tatsuji Nakamura

   Pages 497-514

7. Mass Spectrometry After the Human Genome Project

   • Charles R. Cantor

   Pages 515-529

8. Problems and Prospects in the Characterization of Posttranscriptional Modifications in Large RNAs

   • Pamela F. Crain, Duane E. Ruffner, Yeunghaw Ho, Fenghe Qiu, Jef Rozenski, James A. Mccloskey

   Pages 531-551

9. Back Matter

   Pages 553-577

   PDF

Leading practitioners detail revolutionary new spectrometric techniques for the identification and covalent structural characterization of macromolecules, proteins, glycoconjugates, and nucleic acids. Based on the Fourth International Symposium on Mass Spectrometry in the Health and Life Sciences held in San Francisco in 1998, this invaluable book contains tested strategies for solving many significant biomedical research problems. The techniques use mass spectrometry, automated computer processing of spectral information, and gene, protein, and EST databases for genomic and proteomic correlations. Mass Spectrometry in Biology and Medicine offers a unique opportunity to explore and apply these new techniques of mass spectrometry that are revolutionizing the identification and structural characterization of proteins, carbohydrates, and nucleic acids.

• Polysaccharid
• Proteomics
• RNA
• Translation
• biology
• enzymes
• proteins

". . .of significant reference value. . . "
-Journal of the American Society of Mass Spectrometry

". . .a valuable addition to the library of anyone working in the area of biomolecule characterization by mass spectrometry."
-The Analyst

". . .the writing and explanation are first rate. . ."
-Trends in Analytical Chemistry

"The purpose is to provide an overview of mass spectrometry. This worthy objective has been met. . . In 27 chapters the editors provide a very useful overview of the application of mass spectrometric methods to a wide variety of biological problems."-Doody's Health Sciences Book Review Journal

In 27 chapters the editors provide a very useful overview of the application of mass spectrometric methods to a wide vaiety of biological problems. Examples include determination of purity, molecular weight, presence of posttranslational modifications (glycosylation, phosphorylation), ligand binding, sequencing of peptides, coupling of purification protocols (capillary electrophoresis, for example) and the use of tandem spectrometers.-Doody's Health Sciences Book Review Journal

• University of California, San Francisco, USA

  A. L. Burlingame, Michael A. Baldwin

• SmithKline Beecham Pharmaceuticals, King of Prussia, USA

  Steven A. Carr

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