Skip to main content
  • Book
  • © 1998

Molecular Biomethods Handbook

Part of the book series: Springer Protocols Handbooks (SPH)

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, access via your institution.

Table of contents (51 protocols)

  1. Front Matter

    Pages i-xiii
  2. Extraction of Total RNA from Tissues and Cultured Cells

    • Sandeep Raha, Mingfu Ling, Frank Merante
    Pages 1-8
  3. Isolation of Total Cellular DNA from Tissues and Cultured Cells

    • Frank Merante, Sandeep Raha, Mingfu Ling
    Pages 9-16
  4. Gel Electrophoresis of DNA

    • Duncan R. Smith
    Pages 17-33
  5. Sl Nuclease Analysis of RNA

    • Louis Lefebvre, Stéphane Viville
    Pages 35-49
  6. Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA)

    • Ralf Einspanier, Annette Plath
    Pages 51-57
  7. Gene Probes

    • Marilena Aquino
    Pages 59-72
  8. Nonradioactive Labeling of DNA

    • Thomas P. McCreery, Terrence R. Barrette
    Pages 73-76
  9. Southern Blot Analysis

    • Paolo A. Sabelli
    Pages 77-87
  10. Northern Blot Analysis

    • Paolo A. Sabelli
    Pages 89-93
  11. DNA Sequencing

    • Chris Spencer
    Pages 95-108
  12. Autoradiography and Fluorography

    • Bronwen Harvey
    Pages 109-119
  13. Mobility Shift Assays

    • Nigel J. Savery, Stephen J. W. Busby
    Pages 121-129
  14. cDNA Libraries

    • Ian G.
    Pages 131-143
  15. Genomic DNA Libraries

    • Bimal D. M. Theophilus
    Pages 145-152
  16. λ as a Cloning Vector

    • Rupert Mutzel
    Pages 153-163
  17. Plasmid-Derived Cloning Vectors

    • Craig Winstanley, Ralph Rapley
    Pages 165-179
  18. M13 and Phagemid-Based Cloning Vectors

    • Ralph Rapley
    Pages 181-191
  19. Retroviral Vectors From Laboratory Tools to Molecular Medicines

    • Richard G. Vile, Anna Tuszynski, Simon Castleden
    Pages 193-218
  20. Baculovirus Vectors

    • Azeem Ansari, Vincent C. Emery
    Pages 219-233

About this book

In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included.

The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully. The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.


  • antibody
  • chromosome
  • DNA
  • enzyme
  • flow cytometry
  • gene
  • gene transfer
  • genome
  • glycoprotein
  • monoclonal antibodies
  • RNA
  • tissue
  • transcription


"A good book for those who have been 'brought up' on kits and for those who would like to understand a little bit more about how a technique works rather than simply use it."-Microbiology Today

". . .a good lab investment."-Trends in Genetics

". . .a good teaching resource for undergraduates learning modern molecular genetic techniques. . .provides a clear introduction to many molecular biology techniques. . .best suited to mouse and human genetics."-Molecular Biotechnology

Editors and Affiliations

  • University of Herfordshire, UK

    Ralph Rapley, John M. Walker

Bibliographic Information

  • Book Title: Molecular Biomethods Handbook

  • Editors: Ralph Rapley, John M. Walker

  • Series Title: Springer Protocols Handbooks

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media LLC 1998

  • eBook ISBN: 978-1-59259-642-3Published: 09 October 2007

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XIII, 725

  • Number of Illustrations: 360 b/w illustrations

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access