Molecular Biomethods Handbook

1. Front Matter

   Pages i-xiii

   PDF

2. Extraction of Total RNA from Tissues and Cultured Cells

   • Sandeep Raha, Mingfu Ling, Frank Merante

   Pages 1-8

3. Isolation of Total Cellular DNA from Tissues and Cultured Cells

   • Frank Merante, Sandeep Raha, Mingfu Ling

   Pages 9-16

4. Gel Electrophoresis of DNA

   • Duncan R. Smith

   Pages 17-33

5. Sl Nuclease Analysis of RNA

   • Louis Lefebvre, Stéphane Viville

   Pages 35-49

6. Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA)

   • Ralf Einspanier, Annette Plath

   Pages 51-57

7. Gene Probes

   • Marilena Aquino

   Pages 59-72

8. Nonradioactive Labeling of DNA

   • Thomas P. McCreery, Terrence R. Barrette

   Pages 73-76

9. Southern Blot Analysis

   • Paolo A. Sabelli

   Pages 77-87

10. Northern Blot Analysis

    • Paolo A. Sabelli

    Pages 89-93

11. DNA Sequencing

    • Chris Spencer

    Pages 95-108

12. Autoradiography and Fluorography

    • Bronwen Harvey

    Pages 109-119

13. Mobility Shift Assays

    • Nigel J. Savery, Stephen J. W. Busby

    Pages 121-129

14. cDNA Libraries

    • Ian G.

    Pages 131-143

15. Genomic DNA Libraries

    • Bimal D. M. Theophilus

    Pages 145-152

16. λ as a Cloning Vector

    • Rupert Mutzel

    Pages 153-163

17. Plasmid-Derived Cloning Vectors

    • Craig Winstanley, Ralph Rapley

    Pages 165-179

18. M13 and Phagemid-Based Cloning Vectors

    • Ralph Rapley

    Pages 181-191

19. Retroviral Vectors From Laboratory Tools to Molecular Medicines

    • Richard G. Vile, Anna Tuszynski, Simon Castleden

    Pages 193-218

20. Baculovirus Vectors

    • Azeem Ansari, Vincent C. Emery

    Pages 219-233

In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included.

The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully. The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.

• antibody
• chromosome
• DNA
• enzyme
• flow cytometry
• gene
• gene transfer
• genome
• glycoprotein
• monoclonal antibodies
• RNA
• tissue
• transcription

"A good book for those who have been 'brought up' on kits and for those who would like to understand a little bit more about how a technique works rather than simply use it."-Microbiology Today

". . .a good lab investment."-Trends in Genetics

". . .a good teaching resource for undergraduates learning modern molecular genetic techniques. . .provides a clear introduction to many molecular biology techniques. . .best suited to mouse and human genetics."-Molecular Biotechnology

• University of Herfordshire, UK

  Ralph Rapley, John M. Walker