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Plant and Food Carotenoids

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Manuel Rodríguez-Concepción

    1. CSIC-IRTA-UAB-UB, Centre for Research in Agricultural Genomics (CRAG), Barcelona, Spain

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  2. Ralf Welsch

    1. Institute for Biology II, University of Freiburg, Freiburg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting- edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2083)

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About this book

This volume provides a comprehensive compilation of techniques and protocols used in plant and food carotenoid research. Chapters guide readers through seven major areas on core enzyme activities, analysis of carotenoid profiles, new imaging tools, synthesis and degradation dynamics, biotechnology, nutrition, and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Plant and Food Carotenoids: Methods and Protocols aims to be helpful to researchers of other disciplines that are impacted by carotenoids, including photosynthesis, biotechnology, food science, and nutrition. 

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Pathways for Carotenoid Biosynthesis, Degradation, and Storage

      • Tianhu Sun, Yaakov Tadmor, Li Li
      Pages 3-23

  3. Activities

    1. Front Matter

      Pages 25-25
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    2. A Simple In Vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-Chain Prenyltransferases

      • M. Victoria Barja, Manuel Rodríguez-Concepción
      Pages 27-38

    3. Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase In Vitro

      • Maurizio Camagna, Ralf Welsch
      Pages 39-52

    4. Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO

      • Eleanore T. Wurtzel, Jesús Beltrán
      Pages 53-61

    5. Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases

      • Lourdes Gómez-Gómez, Gianfranco Diretto, Oussama Ahrazem, Salim Al-Babili
      Pages 63-74

    6. Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases

      • Linda Dora Thomas, Srinivasagan Ramkumar, Johannes von Lintig
      Pages 75-88

    7. Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters

      • Olivia Costantina Demurtas, Rita de Brito Francisco, Enrico Martinoia, Giovanni Giuliano
      Pages 89-99

  4. Analytics

    1. Front Matter

      Pages 101-101
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    2. Applications of Visible Spectroscopy and Color Measurements in the Assessments of Carotenoid Levels in Foods

      • Paula Mapelli-Brahm, Francisco J. Rodríguez-Pulido, Carla M. Stinco, Francisco J. Heredia, Antonio J. Meléndez-Martínez
      Pages 103-116

    3. A Routine Method for the Extraction and HPLC-DAD Profiling of Major Plant and Food Carotenoids

      • Dámaso Hornero-Méndez
      Pages 117-134

    4. Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management

      • Antonio Pérez-Gálvez, Isabel Viera, María Roca
      Pages 135-144

    5. cis/trans Carotenoid Extraction, Purification, Detection, Quantification, and Profiling in Plant Tissues

      • Yagiz Alagoz, Namraj Dhami, Chris Mitchell, Christopher I. Cazzonelli
      Pages 145-163

    6. Determination of Plant Volatile Apocarotenoids

      • José L. Rambla, Antonio Granell
      Pages 165-175

    7. A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants

      • Jianing Mi, Kun-Peng Jia, Aparna Balakrishna, Salim Al-Babili
      Pages 177-188

    8. Fractionation of Tomato Fruit Chromoplasts

      • Karel De Pourcq, Albert Boronat
      Pages 189-197

    9. Quantification of Strigolactones

      • Carlos Rial, Rosa M. Varela, José M. G. Molinillo, Alexandra G. Durán, Francisco A. Macías
      Pages 199-208

    10. Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS

      • Daniele Giuffrida, Mariosimone Zoccali, Luigi Mondello
      Pages 209-219
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Editors and Affiliations

  • CSIC-IRTA-UAB-UB, Centre for Research in Agricultural Genomics (CRAG), Barcelona, Spain

    Manuel Rodríguez-Concepción

  • Institute for Biology II, University of Freiburg, Freiburg, Germany

    Ralf Welsch

Bibliographic Information

  • Book Title: Plant and Food Carotenoids

  • Book Subtitle: Methods and Protocols

  • Editors: Manuel Rodríguez-Concepción, Ralf Welsch

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9952-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9951-4Published: 20 November 2019

  • Softcover ISBN: 978-1-4939-9954-5Published: 20 November 2020

  • eBook ISBN: 978-1-4939-9952-1Published: 19 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 421

  • Number of Illustrations: 23 b/w illustrations, 58 illustrations in colour

  • Topics: Plant Sciences, Food Science

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