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Horizontal Gene Transfer

Methods and Protocols

  • Book
  • © 2020


  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2075)

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Table of contents (28 protocols)

  1. Methods for the Detection and Isolation of Mobile Genetic Elements

  2. Methods to Quantify and Characterize Horizontal Gene Transfer


About this book

This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and transduction in HGT as well as a series of methods to analyze the adaptation and evolution of MGEs, with special attention paid to bioinformatics tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Horizontal Gene Transfer: Methods and Protocols serves as an ideal guide to the further study of this pervasive, all-important mechanism of genetic originality.

Editors and Affiliations

  • Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria-CSIC, Santander, Spain

    Fernando de la Cruz

Bibliographic Information

  • Book Title: Horizontal Gene Transfer

  • Book Subtitle: Methods and Protocols

  • Editors: Fernando de la Cruz

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9876-0Published: 04 October 2019

  • Softcover ISBN: 978-1-4939-9879-1Published: 04 October 2020

  • eBook ISBN: 978-1-4939-9877-7Published: 04 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 413

  • Number of Illustrations: 10 b/w illustrations, 52 illustrations in colour

  • Topics: Microbial Genetics and Genomics, Bacteriology

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