Quantitative Real-Time PCR

Methods and Protocols

  • Roberto Biassoni
  • Alessandro Raso

Part of the Methods in Molecular Biology book series (MIMB, volume 2065)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Stephen A. Bustin, Reinhold Mueller, Tania Nolan
    Pages 5-22
  3. Veronika Mussack, Stefanie Hermann, Dominik Buschmann, Benedikt Kirchner, Michael W. Pfaffl
    Pages 23-38
  4. Priscilla Biswas, Eddi Di Marco, Mauro S. Malnati
    Pages 55-64
  5. Gisela Freitas Trindade, Sheila Maria Barbosa de Lima, Constança Britto, Alice Gomes Fernandes-Monteiro
    Pages 65-77
  6. Paula Maza-Márquez, Elisabet Aranda, Jesús González-López, Belén Rodelas
    Pages 79-94
  7. Nicola Luchi, Alberto Santini, Francesca Salvianti, Pamela Pinzani
    Pages 95-104
  8. Ellen De Keyser, Laurence Desmet, Magali Losschaert, Jan De Riek
    Pages 105-118
  9. Danilo J. P. G. Rocha, Thiago L. P. Castro, Eric R. G. R. Aguiar, Luis G. C. Pacheco
    Pages 119-137
  10. Francesca Salvianti, Filomena Costanza, Gemma Sonnati, Pamela Pinzani
    Pages 139-151
  11. Katherine Dominy, Katya Mokretar, Alistair G. Reid, Jamshid S. Khorashad
    Pages 153-173
  12. Ana Paula Santin Bertoni, Isabele Cristiana Iser, Rafael Paschoal de Campos, Márcia Rosangela Wink
    Pages 175-190
  13. Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello et al.
    Pages 191-197
  14. Marion Laig, Christie Fekete, Nivedita Majumdar
    Pages 209-231
  15. Back Matter
    Pages 233-235

About this book


This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study.


Polymerase Chain Reaction RT-qPCR Primer design RT-PCR DNA sequencing qPCR

Editors and affiliations

  • Roberto Biassoni
    • 1
  • Alessandro Raso
    • 2
  1. 1.Molecular DiagnosticsIRCCS, Istituto Giannina GasliniGenovaItaly
  2. 2.ASL3 Sistema Sanitario Regione LiguriaS.C. Laboratorio d’AnalisiGenovaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9832-6
  • Online ISBN 978-1-4939-9833-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site