Yeast Systems Biology

Methods and Protocols

  • Stephen G. Oliver
  • Juan I. Castrillo

Part of the Methods in Molecular Biology book series (MIMB, volume 2049)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Yeast Systems Biology

    1. Front Matter
      Pages 1-1
  3. Experimental Systems Biology: High-Throughput Genome-Wide and Molecular Studies

    1. Front Matter
      Pages 15-15
    2. Sathya Balachander, Taehwan Yang, Gary Newnam, Waleed M. M. El-Sayed, Kyung Duk Koh, Francesca Storici
      Pages 17-37
    3. Jessica M. Walter, Max G. Schubert, Stephanie H. Kung, Kristy Hawkins, Darren M. Platt, Aaron D. Hernday et al.
      Pages 39-72
    4. Katherine Licon, John Paul Shen, Brenton P. Munson, Manuel Michaca, Cole Fassino, Luke Fassino et al.
      Pages 73-85
    5. Deepani W. Poramba-Liyanage, Tessy Korthout, Fred van Leeuwen
      Pages 87-103
    6. Daniel J. H. Nightingale, Stephen G. Oliver, Kathryn S. Lilley
      Pages 165-190
    7. Lucía F. Zacchi, Benjamin L. Schulz
      Pages 191-202
    8. Reza Pourhaghighi, Teresa R. O’Meara, Leah E. Cowen, Andrew Emili
      Pages 203-211
    9. Benedikt M. Beckmann, Sander Granneman
      Pages 213-231
    10. Romanas Chaleckis, Kazuto Ohashi, Isabel Meister, Shama Naz, Craig E. Wheelock
      Pages 233-245
    11. Melody Cools, Maria Rompf, Andreas Mayer, Bruno André
      Pages 247-261
  4. Computational Systems Biology: Data Management and Modeling the Cell

  5. Using Yeast to Model Human Diseases

  6. Back Matter
    Pages 445-447

About this book


This second edition volume expands on the previous edition with a look at the latest advances in techniques to study yeast and its core set of interactions, modules, architectures, and network dynamics that are common in all eukaryotes. The chapters in this book are organized into Four Parts: Part One provides readers with an update on the development of novel experimental and computational approaches to yeast systems biology; Part Two explores high-throughput methods used to study yeast epigenome, transcriptome, proteome, and metabolome; Part Three talks about computational systems biology, and focuses on data management, dynamic modeling, constraint-based models of metabolic networks, and multi-level ‘omics data; while Part Four looks at experimental platforms that utilize yeast to model systemic human diseases such as Alzheimer’s and Parkinson’s diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Yeast System Biology: Methods and Protocols, Second Edition is a valuable tool for graduate students, post-doctoral researchers, and experts who are interested in learning about the latest developments in the study of yeast.


Epigenetics Nucleosome maps ChIP-chip Single-Cell RNA-Seq RNA-splicing

Editors and affiliations

  • Stephen G. Oliver
    • 1
  • Juan I. Castrillo
    • 2
  1. 1.Department of Biochemistry and Cambridge Systems Biology CentreUniversity of CambridgeCambridgeUK
  2. 2.Genetadi Biotech, Parque Tecnológico de BizkaiaBiscaySpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9735-0
  • Online ISBN 978-1-4939-9736-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site