Skip to main content
  • Book
  • © 2019

Cerebrospinal Fluid (CSF) Proteomics

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2044)

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9706-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 149.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xv
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. The Human Brain Proteome Project: Biological and Technological Challenges

      • Joaquín Fernández-Irigoyen, Fernando Corrales, Enrique Santamaría
      Pages 3-23
  3. Cerebrospinal Fluid Sample Preparation Methods for Proteomic Workflows

    1. Front Matter

      Pages 25-25
    2. Guidelines for CSF Processing and Biobanking: Impact on the Identification and Development of Optimal CSF Protein Biomarkers

      • Yanaika S. Hok-A-Hin, Eline A. J. Willemse, Charlotte E. Teunissen, Marta Del Campo
      Pages 27-50
    3. Functional Analyses of Embryonic Cerebrospinal Fluid Proteins

      • Teresa Caprile, Francisco Lamus, María Isabel Alonso, Hernán Montecinos, Angel Gato
      Pages 51-60
    4. CSF Sample Preparation for Data-Independent Acquisition

      • Katalin Barkovits, Lars Tönges, Katrin Marcus
      Pages 61-67
    5. Sample Fractionation Techniques for CSF Peptide Spectral Library Generation

      • Sandra Pacharra, Katrin Marcus, Caroline May
      Pages 69-77
  4. Shotgun Cerebrospinal Fluid Proteomics

    1. Front Matter

      Pages 79-79
    2. Application of 2D-DIGE and iTRAQ Workflows to Analyze CSF in Gliomas

      • Aishwarya A. Rao, Kanika Mehta, Nikita Gahoi, Sanjeeva Srivastava
      Pages 81-110
    3. Peptidomic Workflow Applied to Cerebrospinal Fluid Analysis

      • Rustam H. Ziganshin, Sergey I. Kovalchuk, Igor V. Azarkin
      Pages 111-118
    4. Quantitative Evaluation of Different Protein Fractions of Cerebrospinal Fluid Using 18O Labeling

      • Ramona Birke, Eberhard Krause, Michael Schümann, Ingolf E. Blasig, Reiner F. Haseloff
      Pages 119-128
    5. Determination of Cerebrospinal Fluid Proteome Variations by Isobaric Labeling Coupled with Strong Cation-Exchange Chromatography and Tandem Mass Spectrometry

      • Mercedes Lachén-Montes, Andrea González-Morales, Joaquín Fernández-Irigoyen, Enrique Santamaría
      Pages 155-168
  5. Cerebrospinal Fluid Subproteomics

    1. Front Matter

      Pages 191-191
    2. Top-Down Proteomics Applied to Human Cerebrospinal Fluid

      • Marina Gay, Ester Sánchez-Jiménez, Laura Villarreal, Mar Vilanova, Romain Huguet, Gianluca Arauz-Garofalo et al.
      Pages 193-219
    3. Application of an Aptamer-Based Proteomics Assay (SOMAscan™) in Rat Cerebrospinal Fluid

      • Alba Simats, Laura Ramiro, Joan Montaner, Teresa García-Berrocoso
      Pages 221-231
    4. Monitoring the Cerebrospinal Fluid Cytokine Profile Using Membrane-Based Antibody Arrays

      • Andrea González-Morales, Mercedes Lachén-Montes, Joaquín Fernández-Irigoyen, Enrique Santamaría
      Pages 233-246

About this book

This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other –omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking further insight into this growing field. 

Keywords

  • neuroinflammation
  • Olink technology
  • biomarkers
  • QPRESTs
  • endopeptidome
  • Proteomics

Editors and Affiliations

  • Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIII, Pamplona, Spain

    Enrique Santamaría, Joaquín Fernández-Irigoyen

Bibliographic Information

  • Book Title: Cerebrospinal Fluid (CSF) Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Enrique Santamaría, Joaquín Fernández-Irigoyen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9706-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9705-3

  • Softcover ISBN: 978-1-4939-9708-4

  • eBook ISBN: 978-1-4939-9706-0

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 557

  • Number of Illustrations: 3 b/w illustrations, 164 illustrations in colour

  • Topics: Neuroscience, Protein Biochemistry

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9706-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 149.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)