Overview
- Includes cutting-edge methods and protocols
- provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2044)
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About this book
This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other –omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking furtherinsight into this growing field.
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Keywords
Table of contents (26 protocols)
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Introduction
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Cerebrospinal Fluid Sample Preparation Methods for Proteomic Workflows
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Shotgun Cerebrospinal Fluid Proteomics
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Cerebrospinal Fluid Subproteomics
Editors and Affiliations
Bibliographic Information
Book Title: Cerebrospinal Fluid (CSF) Proteomics
Book Subtitle: Methods and Protocols
Editors: Enrique Santamaría, Joaquín Fernández-Irigoyen
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9706-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-9705-3Published: 21 August 2019
Softcover ISBN: 978-1-4939-9708-4Published: 21 August 2020
eBook ISBN: 978-1-4939-9706-0Published: 20 August 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 557
Number of Illustrations: 3 b/w illustrations, 164 illustrations in colour
Topics: Neurosciences, Neurobiology, Proteomics