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  • © 2019

High-Throughput Protein Production and Purification

Methods and Protocols

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2025)

Buying options

eBook USD 209.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9624-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 269.00
Price excludes VAT (USA)
Hardcover Book USD 379.99
Price excludes VAT (USA)

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Table of contents (24 protocols)

  1. High-Throughput Protein Production Combined with High Throughput Micro-Characterization for Protein Stability, Solubility, Quality and Interactions

    1. A Flexible and Scalable High-Throughput Platform for Recombinant Membrane Protein Production

      • Hui Xu, Thomas Clairfeuille, Christine C. Jao, Hoangdung Ho, Zachary Sweeney, Jian Payandeh et al.
      Pages 389-402
    2. Adaption of the Leishmania Cell-Free Expression System to High-Throughput Analysis of Protein Interactions

      • Wayne A. Johnston, Shayli Varasteh Moradi, Kirill Alexandrov
      Pages 403-421
    3. High-Throughput Protein–Protein Interaction Assays Using Tripartite Split-GFP Complementation

      • Jean-Denis Pedelacq, Geoffrey S. Waldo, Stéphanie Cabantous
      Pages 423-437
    4. High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay

      • Yoan Duhoo, Virginie Girault, Jeremy Turchetto, Laurie Ramond, Fabien Durbesson, Patrick Fourquet et al.
      Pages 439-476
    5. High-Throughput Protein Production Combined with High- Throughput SELEX Identifies an Extensive Atlas of Ciona robusta Transcription Factor DNA-Binding Specificities

      • Kazuhiro R. Nitta, Renaud Vincentelli, Edwin Jacox, Agnès Cimino, Yukio Ohtsuka, Daniel Sobral et al.
      Pages 487-517
    6. High-Throughput Micro-Characterization of RNA–Protein Interactions

      • Sara Gómez, Francisco J. Fernández, M. Cristina Vega
      Pages 519-531
  2. Back Matter

    Pages 533-537

About this book

This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.

Keywords

  • Protein expression
  • Micro-characterization
  • Up-scaling production
  • Soluble yield
  • Structural genomics

Editors and Affiliations

  • Architecture et Fonction des Macromolécules Biologiques (AFMB), Unité Mixte de Recherche (UMR) 7257, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université, Marseille cedex 9, France

    Renaud Vincentelli

Bibliographic Information

  • Book Title: High-Throughput Protein Production and Purification

  • Book Subtitle: Methods and Protocols

  • Editors: Renaud Vincentelli

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9624-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9623-0

  • Softcover ISBN: 978-1-4939-9626-1

  • eBook ISBN: 978-1-4939-9624-7

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 537

  • Number of Illustrations: 39 b/w illustrations, 106 illustrations in colour

  • Topics: Protein Biochemistry

Buying options

eBook USD 209.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9624-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 269.00
Price excludes VAT (USA)
Hardcover Book USD 379.99
Price excludes VAT (USA)