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  • © 2019

High-Throughput Protein Production and Purification

Methods and Protocols

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2025)

Buying options

eBook USD 209.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9624-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 269.00
Price excludes VAT (USA)
Hardcover Book USD 379.99
Price excludes VAT (USA)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xvi
  2. General Procedures for High-Throughput Protein Production

    1. Front Matter

      Pages 1-1
    2. Overview of High-Throughput Cloning Methods for the Post-genomic Era

      • Claudia Ortega, Cecilia Abreu, Pablo Oppezzo, Agustín Correa
      Pages 3-32
    3. Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins

      • Joanne E. Nettleship, Heather Rada, Raymond J. Owens
      Pages 33-49
    4. Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins

      • Edward Kraft, Yvonne Franke, Katharine Heeringa, Stephanie Shriver, Inna Zilberleyb, Christine Kugel et al.
      Pages 51-68
    5. High-Throughput Protein Production in Yeast

      • Francisco J. Fernández, Sara Gómez, M. Cristina Vega
      Pages 69-91
    6. A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells

      • Sarah M. Rue, Paul W. Anderson, Michelle R. Gaylord, Jessica J. Miller, Scott M. Glaser, Scott A. Lesley
      Pages 93-142
    7. A High-Throughput Automated Protein Folding System

      • Kenneth W. Walker, Philip An, Dwight Winters
      Pages 143-161
  3. High-Throughput Protocols Adapted to the Production of Specific Protein Families

    1. Front Matter

      Pages 163-163
    2. High-Throughput Production of Oxidized Animal Toxins in Escherichia coli

      • Yoan Duhoo, Ana Filipa Sequeira, Natalie J. Saez, Jeremy Turchetto, Laurie Ramond, Fanny Peysson et al.
      Pages 165-190
    3. High-Throughput Purification of Protein Kinases from Escherichia coli and Insect Cells

      • Sebastian Mathea, Eidarus Salah, Stefan Knapp
      Pages 191-202
    4. Parallelized Microscale Expression of Soluble scFv

      • Giulio Russo, Viola Fühner, André Frenzel, Michael Hust, Stefan Dübel
      Pages 203-211
    5. High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression

      • Duygu Sari-Ak, Shervin Bahrami, Magdalena J. Laska, Petra Drncova, Daniel J. Fitzgerald, Christiane Schaffitzel et al.
      Pages 213-226
    6. High-Throughput E. coli Cell-Free Expression: From PCR Product Design to Functional Validation of GPCR

      • Sandra Cortès, Fatima-Ezzahra Hibti, Frydman Chiraz, Safia Ezzine
      Pages 261-279
    7. High-Throughput Site-Directed Mutagenesis

      • Claire Strain-Damerell, Nicola A. Burgess-Brown
      Pages 281-296
  4. High-Throughput Protein Production Combined with High Throughput Micro-Characterization for Protein Stability, Solubility, Quality and Interactions

    1. Front Matter

      Pages 297-297
    2. High-Throughput Isolation of Soluble Protein Domains Using a Bipartite Split-GFP Complementation System

      • Amélie Massemin, Stéphanie Cabantous, Geoffrey S. Waldo, Jean-Denis Pedelacq
      Pages 321-333

About this book

This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.

Keywords

  • Protein expression
  • Micro-characterization
  • Up-scaling production
  • Soluble yield
  • Structural genomics

Editors and Affiliations

  • Architecture et Fonction des Macromolécules Biologiques (AFMB), Unité Mixte de Recherche (UMR) 7257, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université, Marseille cedex 9, France

    Renaud Vincentelli

Bibliographic Information

  • Book Title: High-Throughput Protein Production and Purification

  • Book Subtitle: Methods and Protocols

  • Editors: Renaud Vincentelli

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9624-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9623-0

  • Softcover ISBN: 978-1-4939-9626-1

  • eBook ISBN: 978-1-4939-9624-7

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 537

  • Number of Illustrations: 39 b/w illustrations, 106 illustrations in colour

  • Topics: Protein Biochemistry

Buying options

eBook USD 209.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9624-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 269.00
Price excludes VAT (USA)
Hardcover Book USD 379.99
Price excludes VAT (USA)