Editors:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1989)
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Table of contents (21 protocols)
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Front Matter
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Setting Up a Facility
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Front Matter
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Panel Design and Reagent Preparation
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Front Matter
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Sample Preparation
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Front Matter
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Specific Applications
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Front Matter
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About this book
This book details up-to-date and cutting edge compilation of protocols in mass cytometry. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Keywords
- multiparametric data
- panel designs
- phenotyping
- mouse samples
- Nanoparticles
- antibodies
Editors and Affiliations
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Discipline of Pathology, The University of Sydney, Camperdown, Australia
Helen M. McGuire
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Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Camperdown, Australia
Thomas M. Ashhurst
Bibliographic Information
Book Title: Mass Cytometry
Book Subtitle: Methods and Protocols
Editors: Helen M. McGuire, Thomas M. Ashhurst
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9454-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-9453-3Published: 13 May 2019
eBook ISBN: 978-1-4939-9454-0Published: 10 May 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 338
Number of Illustrations: 13 b/w illustrations, 70 illustrations in colour
Topics: Cell Biology, Mass Spectrometry, Antibodies