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  • © 2019

Experimental Cholestasis Research

Editors:

(view affiliations)
  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1981)

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USD 109.00
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  • ISBN: 978-1-4939-9420-5
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  • Tax calculation will be finalised during checkout
Softcover Book
USD 149.99
Price excludes VAT (USA)
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Price excludes VAT (USA)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
  2. Mechanisms of Drug-Induced Cholestasis

    • Eva Gijbels, Mathieu Vinken
    Pages 1-14
  3. LC-MS/MS Analysis of Bile Acids in In Vitro Samples

    • Debbie Dewaele, Pieter Annaert, Eef Hoeben
    Pages 15-23
  4. Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis

    • Amruta Damle-Vartak, Brigitte Begher-Tibbe, Georgia Gunther, Fabian Geisler, Nachiket Vartak, Jan G. Hengstler
    Pages 25-53
  5. Vesicle- and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function

    • Pieter Van Brantegem, Neel Deferm, Bing Qi, Tom De Vocht, Pieter Annaert
    Pages 55-73
  6. In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids

    • Stef De Lombaerde, Sara Neyt, Christian Vanhove, Filip De Vos
    Pages 87-98
  7. Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy

    • Tânia Sousa, Rui E. Castro, Ana Coutinho, Cecília M. P. Rodrigues, Manuel Prieto, Fábio Fernandes
    Pages 99-115
  8. Measuring Apoptosis and Necrosis in Cholestatic Liver Injury

    • Benjamin L. Woolbright, Hartmut Jaeschke
    Pages 133-147
  9. Necrosome Formation and Necroptosis in Experimental Cholestasis

    • Marta B. Afonso, Cecília M. P. Rodrigues
    Pages 149-162
  10. Proteomics in Primary Biliary Cholangitis

    • You Li, Ruqui Tang, Patrick S. C. Leung, M. Eric Gershwin, Xiong Ma
    Pages 163-173
  11. Transcriptomic Analysis of Cholestatic Compounds In Vitro

    • Céline Parmentier, Philippe Couttet, Marianne Uteng, Armin Wolf, Lysiane Richert
    Pages 175-186
  12. System Microscopy of Stress Response Pathways in Cholestasis Research

    • Johannes P. Schimming, Bas ter Braak, Marije Niemeijer, Steven Wink, Bob van de Water
    Pages 187-202
  13. Mouse Models for Diseases in the Cholangiocyte Lineage

    • Noémi Van Hul, Urban Lendahl, Emma R. Andersson
    Pages 203-236
  14. Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis

    • Sanne Van Campenhout, Hans Van Vlierberghe, Lindsey Devisscher
    Pages 237-247
  15. A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis

    • Sujit K. Mohanty, Bryan Donnelly, Haley Temple, Gregory M. Tiao
    Pages 259-271
  16. Zebrafish as a Model to Study Cholestatic Liver Diseases

    • Duc-Hung Pham, Chunyue Yin
    Pages 273-289
  17. Setup and Use of HepaRG Cells in Cholestasis Research

    • Christiane Guguen-Guillouzo, André Guillouzo
    Pages 291-312

About this book

This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic and applied researchers pursuing this vital area of research in pharmacology and toxicology.

Keywords

  • Drug-induced liver injury
  • Liver toxicology
  • DILI
  • Cholestatic drug properties
  • Model organisms
  • Impaired bile secretion
  • Hepatocellular injury
  • hepatology

Editors and Affiliations

  • Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium

    Mathieu Vinken

Bibliographic Information

  • Book Title: Experimental Cholestasis Research

  • Editors: Mathieu Vinken

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9420-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9419-9

  • Softcover ISBN: 978-1-4939-9422-9

  • eBook ISBN: 978-1-4939-9420-5

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 399

  • Number of Illustrations: 14 b/w illustrations, 60 illustrations in colour

  • Topics: Pharmacology, Internal Medicine

Buying options

eBook
USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9420-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 149.99
Price excludes VAT (USA)
Hardcover Book
USD 219.99
Price excludes VAT (USA)