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Neisseria meningitidis

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Kate L. Seib

    1. Institute For Glycomics, Griffith University, Southport, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Ian R. Peak

    1. Institute For Glycomics, Griffith University, Southport, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1969)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. An Overview of Neisseria meningitidis

    • Sarah Hollingshead, Christoph M. Tang
    Pages 1-16

  3. Diagnosis of Meningococcal Infection Using Internally Controlled Multiplex Real-Time PCR

    • Ala-Eddine Deghmane, Eva Hong, Muhamed-Kheir Taha
    Pages 17-31

  4. Investigating RNA–Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis

    • Nadja Heidrich, Saskia Bauriedl, Christoph Schoen
    Pages 33-49

  5. Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics

    • Holly B. Bratcher, Odile B. Harrison, Martin C. J. Maiden
    Pages 51-82

  6. Determination of Repeat Number and Expression States of Phase-Variable Loci Through Next Generation Sequencing and Bioinformatic Analysis

    • Luke R. Green, Richard D. Haigh, Christopher D. Bayliss
    Pages 83-92

  7. Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis

    • Freda E. -C. Jen, Kate L. Seib, Aimee Tan
    Pages 93-104

  8. Sample Preparation for Proteomic Analysis of Neisseria meningitidis

    • Benjamin L. Schulz
    Pages 105-112

  9. Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance

    • Tsitsi D. Mubaiwa, Lauren E. Hartley-Tassell, Evgeny A. Semchenko, Christopher J. Day, Michael P. Jennings, Kate L. Seib
    Pages 113-121

  10. The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp.

    • Emily A. Kibble, Mitali Sarkar-Tyson, Geoffrey W. Coombs, Charlene M. Kahler
    Pages 123-133

  11. In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells

    • Brandon J. Kim, Alexandra Schubert-Unkmeir
    Pages 135-148

  12. Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease

    • Kay O. Johswich, Scott D. Gray-Owen
    Pages 149-168

  13. Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays

    • Jay Lucidarme, Jennifer Louth, Kelly Townsend-Payne, Ray Borrow
    Pages 169-179

  14. Monoclonal Antibodies Against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitidis: A Platform for the Quality Control of Meningococcal Vaccines

    • Elizabeth González, Fátima Reyes, Oscar Otero, Frank Camacho, Maribel Cuello, Fidel Ramírez et al.
    Pages 181-203

  15. Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine

    • Giuseppe Boccadifuoco, Brunella Brunelli, Elena Mori, Mauro Agnusdei, Claudia Gianfaldoni, Marzia Monica Giuliani
    Pages 205-215

  16. Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitidis

    • Jakob Loschko, Karen Garcia, David Cooper, Michael Pride, Annaliesa Anderson
    Pages 217-236

  17. Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis

    • Caroline Cayrou, Christopher D. Bayliss
    Pages 237-249

  18. Back Matter

    Pages 251-253
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Keywords

About this book

This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration andconsistency in investigations of this enigmatic and dangerous pathogen.

Editors and Affiliations

  • Institute For Glycomics, Griffith University, Southport, Australia

    Kate L. Seib, Ian R. Peak

Bibliographic Information

  • Book Title: Neisseria meningitidis

  • Book Subtitle: Methods and Protocols

  • Editors: Kate L. Seib, Ian R. Peak

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9202-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9201-0Published: 16 March 2019

  • eBook ISBN: 978-1-4939-9202-7Published: 15 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 253

  • Number of Illustrations: 16 b/w illustrations, 42 illustrations in colour

  • Topics: Bacteriology, Vaccine, Infectious Diseases

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