© 2019


Methods and Protocols

  • Mark G. H. Scott
  • Stéphane A. Laporte
  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1957)

Table of contents

  1. Methods to Study β-Arrestin Localization

    1. Front Matter
      Pages 249-249
    2. Elodie Blondel-Tepaz, Thomas Guilbert, Mark G. H. Scott
      Pages 251-269
    3. Anahi Molla-Herman, Kathryn M. Davis, Kirk Mykytyn, Alexandre Benmerah
      Pages 271-289
  2. Methods to Study Conformational Change of β-Arrestin

    1. Front Matter
      Pages 291-291
    2. Erik G. Strungs, Louis M. Luttrell, Mi-Hye Lee
      Pages 309-322
  3. Methods to Study Roles of β-Arrestins in Physiopathology

    1. Front Matter
      Pages 323-323
    2. Zoé Virion, Stefano Marullo, Mathieu Coureuil
      Pages 325-334
    3. Stéphane Dalle, Safia Costes, Gyslaine Bertrand, Magalie A. Ravier
      Pages 345-364
    4. Bing Luan, Jian Zhao, Gang Pei
      Pages 365-384
    5. Laura Rosanò, Roberta Cianfrocca, Anna Bagnato
      Pages 393-406
  4. Back Matter
    Pages 407-409

About this book


This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in  MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson’s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins.


cell signalling GPCR cAMP cell migration cell adhesion Receptors

Editors and affiliations

  • Mark G. H. Scott
    • 1
  • Stéphane A. Laporte
    • 2
  1. 1.Institut CochinINSERM U1016ParisFrance
  2. 2.Department of Medicine, Research Institute of the McGillUniversity Health Center (RI-MUHC), McGill UniversityMontrealCanada

Bibliographic information

  • Book Title Beta-Arrestins
  • Book Subtitle Methods and Protocols
  • Editors Mark G. H. Scott
    Stéphane A. Laporte
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9157-0
  • eBook ISBN 978-1-4939-9158-7
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 409
  • Number of Illustrations 19 b/w illustrations, 60 illustrations in colour
  • Topics Protein Science
    Cell Biology
  • Buy this book on publisher's site