© 2019


Methods and Protocols

  • Mark G. H. Scott
  • Stéphane A. Laporte
  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1957)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Marc G. Caron, Lawrence S. Barak
      Pages 3-8
  3. Methods to Study Roles of β-Arrestins in GPCR Regulation

  4. Methods to Study Scaffolding Functions of β-Arrestins

    1. Front Matter
      Pages 105-105
    2. Nicole A. Perry, Xuanzhi Zhan, Eugenia V. Gurevich, T. M. Iverson, Vsevolod V. Gurevich
      Pages 107-120
    3. Amy J. Tibbo, Tara Busiau, George S. Baillie
      Pages 121-137
    4. Revu Ann Alexander, Isaure Lot, Hervé Enslen
      Pages 139-158
    5. Thomas Bourquard, Astrid Musnier, Aurélie Tréfier, Flavie Landomiel, Thomas Boulo, Eric Reiter et al.
      Pages 177-194
    6. Romain Yvinec, Mohammed Akli Ayoub, Francesco De Pascali, Pascale Crépieux, Eric Reiter, Anne Poupon
      Pages 195-215
    7. Yang Zhao, Kunhong Xiao
      Pages 217-232
  5. Methods to Analyse Ligand Bias

    1. Front Matter
      Pages 233-233
    2. Edward L. Stahl, Frederick J. Ehlert, Laura M. Bohn
      Pages 235-247

About this book


This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in  MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson’s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins.


cell signalling GPCR cAMP cell migration cell adhesion Receptors

Editors and affiliations

  • Mark G. H. Scott
    • 1
  • Stéphane A. Laporte
    • 2
  1. 1.Institut CochinINSERM U1016ParisFrance
  2. 2.Department of Medicine, Research Institute of the McGillUniversity Health Center (RI-MUHC), McGill UniversityMontrealCanada

Bibliographic information

  • Book Title Beta-Arrestins
  • Book Subtitle Methods and Protocols
  • Editors Mark G. H. Scott
    Stéphane A. Laporte
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9157-0
  • eBook ISBN 978-1-4939-9158-7
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 409
  • Number of Illustrations 19 b/w illustrations, 60 illustrations in colour
  • Topics Protein Science
    Cell Biology
  • Buy this book on publisher's site