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Intracellular Lipid Transport

Methods and Protocols

  • Guillaume Drin

Part of the Methods in Molecular Biology book series (MIMB, volume 1949)

Table of contents

  1. Front Matter
    Pages i-xi
  2. József T. Tóth, Gergő Gulyás, László Hunyady, Péter Várnai
    Pages 23-34
  3. Juan Martín D’Ambrosio, Véronique Albanèse, Alenka Čopič
    Pages 35-46
  4. Serena Capasso, Giovanni D’Angelo
    Pages 47-56
  5. Juliette Jouhet, Valérie Gros, Morgane Michaud
    Pages 69-93
  6. Anders P. E. Backman, Josefin Halin, Matti A. Kjellberg, Peter Mattjus
    Pages 105-114
  7. Neha Chauhan, Julian A. Jentsch, Anant K. Menon
    Pages 115-136
  8. Léa P. Wilhelm, Laetitia Voilquin, Toshihide Kobayashi, Catherine Tomasetto, Fabien Alpy
    Pages 137-152
  9. Shreya Endapally, Rodney E. Infante, Arun Radhakrishnan
    Pages 153-163
  10. Roopa Kenoth, Rhoderick E. Brown, Ravi Kanth Kamlekar
    Pages 237-256
  11. Back Matter
    Pages 341-345

About this book

Introduction

This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or at membrane contact sites, or within cellular membranes. The volume begins with methodologies to measure the movement of varied lipid species between or in organelle membranes, inside eukaryotic cells, including plant cells, or in bacteria, and continues in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoints, how lipid transfer proteins (LTPs) or flippases/scramblases precisely function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Intracellular Lipid Transport: Methods and Protocols serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cellular process.

Keywords

Lipid transfer proteins (LTPs) Membrane contact sites Sphingolipids Glycerophospholipids Organelle membranes Flippases/scramblases

Editors and affiliations

  • Guillaume Drin
    • 1
  1. 1.Institut de Pharmacologie Moléculaire et CellulaireUniversité Côte d’Azur and CNRSValbonneFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9136-5
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9135-8
  • Online ISBN 978-1-4939-9136-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site