Advertisement

Tumor Profiling

Methods and Protocols

  • Sarah S. Murray

Part of the Methods in Molecular Biology book series (MIMB, volume 1908)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Eric Strengman, Francoise A. S. Barendrecht-Smouter, Carmen de Voijs, Paula de Vree, Isaac J. Nijman, Wendy W. J. de Leng
    Pages 1-17
  3. Guy Froyen, Brigitte Maes
    Pages 73-87
  4. Wei Shen, Philippe Szankasi, Jacob Durtschi, Todd W. Kelley, Xinjie Xu
    Pages 113-124
  5. Jan Schröder, Amit Kumar, Stephen Q. Wong
    Pages 125-138
  6. Sean M. Courtney, Willian A. da Silveira, E. Starr Hazard, Gary Hardiman
    Pages 169-184
  7. Willian A. da Silveira, E. Starr Hazard, Dongjun Chung, Gary Hardiman
    Pages 185-204
  8. Teresia Kling, Helena Carén
    Pages 205-217
  9. Arundhati Bakshi, Muhammad B. Ekram, Joomyeong Kim
    Pages 219-228
  10. Jana- A. Thiele, Pavel Pitule, James Hicks, Peter Kuhn
    Pages 243-264
  11. Back Matter
    Pages 265-268

About this book

Introduction

This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenetic changes using both next-generation sequencing and microarray techniques, common bioinformatics and annotation tools to glean relevant driver genomic events, and different performance characteristics as well as quality metrics necessary for the robust validation of tumor profiling as a diagnostic test for medical laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Tumor Profiling: Methods and Protocols aims to be a useful resource for learning about technical details, applications, and strengths and limitations of the latest technologies as applied to this increasingly important field. 


Keywords

NGS STRs genomic alterations heterogeneity qPCR

Editors and affiliations

  • Sarah S. Murray
    • 1
  1. 1.Department of PathologyUniversity of California San DiegoLa JollaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9004-7
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9002-3
  • Online ISBN 978-1-4939-9004-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site