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Cancer Driver Genes

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Timothy K. Starr

    1. Masonic Cancer Center, University of Minnesota Medical School, Minneapolis, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1907)

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About this book

This book presents protocols for identification of genetic drivers of cancer. Chapters guide readers through a brief history of cancer gene discovery, in silico approaches, in vitro approaches, and in vivo approaches using forward genetic screens in mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cancer Driver Genes: Methods and Protocols aims to provide protocols that will be used and adapted by cancer researchers to expand the knowledge base of molecular mechanisms contributing to initiation, progression, and metastasis of cancer.

 

 

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Cancer Gene Discovery: Past to Present

    • Christopher R. Clark, Wilaiwan DuRose, Timothy K. Starr
    Pages 1-15

  4. Cancer Gene Discovery Using Cell and Organoid Systems

    1. Front Matter

      Pages 81-81
      Download chapter PDF

    2. A Cell-Based Method for Identification of Chemotherapy Resistance Cancer Genes

      • Raffaele Hellweg, Ashley Mooneyham, Martina Bazzaro
      Pages 83-90

    3. Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization

      • Huanhuan Joyce Chen, Michael L. Shuler
      Pages 91-102

    4. Mutagenesis Screens for Prostate Cancer Using Replication-Incompetent Lentiviral Vectors

      • Grant D. Trobridge
      Pages 103-114

    5. Arrayed shRNA Screening to Identify Suppressors of Anchorage-Independent Growth

      • Ugur Eskiocak
      Pages 115-123

    6. Genome-Wide CRISPR/Cas9 Screening for Identification of Cancer Genes in Cell Lines

      • Charles H. Adelmann, Tim Wang, David M. Sabatini, Eric S. Lander
      Pages 125-136

    7. CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits

      • Nicholas J. Slipek, Jyotika Varshney, David A. Largaespada
      Pages 137-144

    8. Ex Vivo Transposon-Mediated Genetic Screens for Cancer Gene Discovery

      • Kathryn A. O’Donnell, Yabin Guo, Shruthy Suresh, Barrett L. Updegraff, Xiaorong Zhou
      Pages 145-157

  5. Cancer Gene Discovery Using Animal Models

    1. Front Matter

      Pages 159-159
      Download chapter PDF

    2. Cancer Gene Discovery Utilizing Sleeping Beauty Transposon Mutagenesis

      • Kelsie L. Becklin, Branden A. Smeester, Branden S. Moriarity
      Pages 161-170

    3. PiggyBac Transposon-Based Insertional Mutagenesis in Mice

      • Mathias J. Friedrich, Iraad F. Bronner, Pentao Liu, Allan Bradley, Roland Rad
      Pages 171-183

    4. Liver-Specific Delivery of Sleeping Beauty Transposon System by Hydrodynamic Injection for Cancer Gene Validation

      • Amy P. Chiu, Vincent W. Keng
      Pages 185-196

    5. Engineering Large Genomic Rearrangement in Mouse Embryonic Stem Cell for Cancer Gene Discovery

      • Yuen-Yi Tseng, Anindya Bagchi
      Pages 197-212
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Editors and Affiliations

  • Masonic Cancer Center, University of Minnesota Medical School, Minneapolis, USA

    Timothy K. Starr

Bibliographic Information

  • Book Title: Cancer Driver Genes

  • Book Subtitle: Methods and Protocols

  • Editors: Timothy K. Starr

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8967-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8966-9Published: 13 December 2018

  • eBook ISBN: 978-1-4939-8967-6Published: 13 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 214

  • Number of Illustrations: 11 b/w illustrations, 35 illustrations in colour

  • Topics: Cancer Research, Human Genetics

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