Immunological Tolerance

Methods and Protocols

  • Ashleigh S. Boyd

Part of the Methods in Molecular Biology book series (MIMB, volume 1899)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Isolation of Stem Cells and Their Progeny for Immunological Tolerance Applications

    1. Front Matter
      Pages 1-1
    2. Anna Wilson, Melisse Chee, Peter Butler, Ashleigh S. Boyd
      Pages 3-13
    3. Juan Bautista Menendez-Gonzalez, Lubaid Saleh, Gui Jie Feng, Neil P. Rodrigues
      Pages 15-23
    4. Toshinobu Nishimura, Hiromitsu Nakauchi
      Pages 25-40
  3. Isolation of Regulatory Lymphocyte Populations

    1. Front Matter
      Pages 41-41
    2. Kate Milward, Joanna Hester, Kathryn J. Wood
      Pages 43-54
    3. Madhvi Menon, Elizabeth C. Rosser, Claudia Mauri
      Pages 55-66
  4. Mechanisms and Induction of Immunological Tolerance

  5. Transplantation Tolerance

  6. The Breakdown of Immunological Tolerance in Disease

  7. Back Matter
    Pages 239-240

About this book


This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field.


tolerogenic cell tolerogenic lymphocytic cells B regulatory populations thymus progenitors stem cells self-renewing cell

Editors and affiliations

  • Ashleigh S. Boyd
    • 1
  1. 1.Associate Professor in Stem Cell Biology and Regenerative Medicine, Research Department of Surgical Biotechnology, Division of Surgery and Interventional ScienceUniversity College London (UCL), Royal Free HospitalLondonUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8936-2
  • Online ISBN 978-1-4939-8938-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site