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  • © 2019

SNAREs

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1860)

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-8760-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
  2. Biophysics and Computational Analysis

    1. Front Matter

      Pages 1-1
    2. EPR Lineshape Analysis to Investigate the SNARE Folding Intermediates

      • Ryan Khounlo, Brenden J. D. Hawk, Yeon-Kyun Shin
      Pages 33-51
    3. SNAREpin Assembly: Kinetic and Thermodynamic Approaches

      • Feng Li, Frederic Pincet
      Pages 71-93
    4. Single-Molecule Optical Tweezers Study of Regulated SNARE Assembly

      • Lu Ma, Junyi Jiao, Yongli Zhang
      Pages 95-114
    5. Studying Munc18:Syntaxin Interactions Using Small-Angle Scattering

      • Andrew E. Whitten, Russell J. Jarrott, Shu-Hong Hu, Anthony P. Duff, Gordon J. King, Jennifer L. Martin et al.
      Pages 115-144
  3. Biochemistry

    1. Front Matter

      Pages 161-161
    2. SNAP-25 S-Guanylation and SNARE Complex Formation

      • Yusuke Kishimoto, Takaaki Akaike, Hideshi Ihara
      Pages 163-173
    3. Analysis of the Role of Sec3 in SNARE Assembly and Membrane Fusion

      • Kunrong Mei, Peng Yue, Wei Guo
      Pages 175-189
    4. Determination of Sec18-Lipid Interactions by Liposome-Binding Assay

      • Matthew L. Starr, Rutilio Fratti
      Pages 211-220
    5. Using Nanodiscs to Probe Ca2+-Dependent Membrane Interaction of Synaptotagmin-1

      • Ekaterina Stroeva, Shyam S. Krishnakumar
      Pages 221-236
    6. Functional Reconstitution of Intracellular Vesicle Fusion Using Purified SNAREs and Sec1/Munc18 (SM) Proteins

      • Haijia Yu, Lauren Crisman, Michael H. B. Stowell, Jingshi Shen
      Pages 237-249
  4. Functional Assays

    1. Front Matter

      Pages 251-251
    2. Assay of Lipid Mixing and Fusion Pore Formation in the Fusion of Yeast Vacuoles

      • Massimo D’Agostino, Andreas Mayer
      Pages 253-262

About this book

This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. The methods described in each chapter are described in detail so that novice as well as experienced researchers can explore the mechanisms of SNARE-mediated membrane fusion. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17, examining the structures and interactions of SNAREs, measuring the interactions of SNAREs, interactions of SNAREs, and post-translational modifications of SNAREs and how they affect function.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, SNAREs: Methods and Protocols aims to be a valuable tool for all investigators interested in the field.

Keywords

  • Eukaryotic cells
  • membrane trafficking
  • post-translational modifications
  • Reconstituted proteoliposomes
  • Liposomes

Editors and Affiliations

  • Department of Biochemistry, University of Illinois Urbana-Champaign, Urbana, USA

    Rutilio Fratti

Bibliographic Information

  • Book Title: SNAREs

  • Book Subtitle: Methods and Protocols

  • Editors: Rutilio Fratti

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8760-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8759-7

  • Softcover ISBN: 978-1-4939-9385-7

  • eBook ISBN: 978-1-4939-8760-3

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 407

  • Number of Illustrations: 8 b/w illustrations, 66 illustrations in colour

  • Topics: Biochemistry

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-8760-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)