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Recombinant Protein Expression in Mammalian Cells

Methods and Protocols

  • Book
  • © 2018

Overview

  • Includes cutting-edge methods and protocols for the study of recombinant protein expression
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1850)

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About this book

This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host;  however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.

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Keywords

Table of contents (19 protocols)

Editors and Affiliations

  • Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

    David L. Hacker

Bibliographic Information

  • Book Title: Recombinant Protein Expression in Mammalian Cells

  • Book Subtitle: Methods and Protocols

  • Editors: David L. Hacker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8730-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8729-0Published: 22 September 2018

  • Softcover ISBN: 978-1-4939-9377-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8730-6Published: 21 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 311

  • Number of Illustrations: 8 b/w illustrations, 68 illustrations in colour

  • Topics: Protein Science

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