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Plastids

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Eric Maréchal

    1. Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l’Energie Atomique et aux Energies Alternatives, CEA Grenoble, Institut National Recherche Agronomique, UMR5168, Université Grenoble Alpes, Grenoble, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1829)

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Table of contents (26 protocols)

  1. Laboratory Protocols

    1. Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction

      • Florence Courtois, Livia Merendino
      Pages 273-278

    2. Bioinformatic Analysis of Chloroplast Gene Expression and RNA Posttranscriptional Maturations Using RNA Sequencing

      • Bastien Malbert, Guillem Rigaill, Veronique Brunaud, Claire Lurin, Etienne Delannoy
      Pages 279-294

    3. A Guide to the Chloroplast Transcriptome Analysis Using RNA-Seq

      • Elena J. S. Michel, Amber M. Hotto, Susan R. Strickler, David B. Stern, Benoît Castandet
      Pages 295-313

    4. A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae

      • M. Águila Ruiz-Sola, Dimitris Petroutsos
      Pages 315-323

    5. Rescue of Deletion Mutants to Isolate Plastid Transformants in Higher Plants

      • Mohammad El Hajj, Mohd Fahdli Bin Hamdan, Elena Martin Avila, Anil Day
      Pages 325-339

    6. Genetic Analysis of Chloroplast Biogenesis, and Function and Mutant Collections

      • Sho Fujii, Hajime Wada, Koichi Kobayashi
      Pages 341-365

    7. Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum

      • Guillaume Allorent, Erika Guglielmino, Cécile Giustini, Florence Courtois
      Pages 367-378

  2. In Silico Tools

    1. Front Matter

      Pages 379-379
      Download chapter PDF

    2. In Silico Tools for the Prediction of Protein Import into Secondary Plastids

      • Daniel Moog
      Pages 381-394

    3. AT_CHLORO: The First Step When Looking for Information About Subplastidial Localization of Proteins

      • Daniel Salvi, Sylvain Bournais, Lucas Moyet, Imen Bouchnak, Marcel Kuntz, Christophe Bruley et al.
      Pages 395-406

  3. Back Matter

    Pages 407-409
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Keywords

About this book

This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The chapters in this book also look at various techniques to analyze plastids through means of combining biology strategies from genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and thorough, Plastids: Methods and Protocols is a valuable resource for students, engineers, and researchers who are interested in this evolving organelle and overall field.

Editors and Affiliations

  • Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l’Energie Atomique et aux Energies Alternatives, CEA Grenoble, Institut National Recherche Agronomique, UMR5168, Université Grenoble Alpes, Grenoble, France

    Eric Maréchal

Bibliographic Information

  • Book Title: Plastids

  • Book Subtitle: Methods and Protocols

  • Editors: Eric Maréchal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8654-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8653-8Published: 10 July 2018

  • Softcover ISBN: 978-1-4939-9355-0Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8654-5Published: 09 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 409

  • Number of Illustrations: 20 b/w illustrations, 46 illustrations in colour

  • Topics: Plant Sciences

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