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  • © 2018

Plastids

Methods and Protocols

Editors:

  1. Eric Maréchal

    1. Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l’Energie Atomique et aux Energies Alternatives, CEA Grenoble, Institut National Recherche Agronomique, UMR5168, Université Grenoble Alpes, Grenoble, France

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  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1829)

Sections

eBook USD 169.00
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  • ISBN: 978-1-4939-8654-5
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Softcover Book USD 219.99
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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xv
    PDF

  2. Plastid Evolution, Plasticity, Structural, and Functional Diversity

    1. Front Matter

      Pages 1-1
      PDF

    5. Diversity and Plasticity of Plastids in Land Plants

      • Katalin Solymosi, Johanna Lethin, Henrik Aronsson
      Pages 55-72

    6. The Main Functions of Plastids

      • Norbert Rolland, Imen Bouchnak, Lucas Moyet, Daniel Salvi, Marcel Kuntz
      Pages 73-85

    7. Plastid Transient and Stable Interactions with Other Cell Compartments

      • Stefanie J. Mueller-Schuessele, Morgane Michaud
      Pages 87-109

  3. Laboratory Protocols

    1. Front Matter

      Pages 111-111
      PDF

    2. Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy

      • Serena Flori, Pierre-Henri Jouneau, Benoit Gallet, Leandro F. Estrozi, Christine Moriscot, Guy Schoehn et al.
      Pages 113-122

    8. Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum

      • Alexander F. Schober, Serena Flori, Giovanni Finazzi, Peter G. Kroth, Carolina Río Bártulos
      Pages 189-203

    9. Isolating the Plasmodium falciparum Apicoplast Using Magnetic Beads

      • Cyrille Y. Botté, Geoffrey I. McFadden, Yoshiki Yamaryo-Botté
      Pages 205-212

    10. Extraction and Quantification of Lipids from Plant or Algae

      • Valérie Gros, Josselin Lupette, Juliette Jouhet
      Pages 213-240

    11. Quantitative Assessment of the Chloroplast Lipidome

      • Valérie Gros, Juliette Jouhet
      Pages 241-252

    12. Determination of the DNA/RNA-Associated Subproteome from Chloroplasts and Other Plastid Types

      • Maha Chieb, Monique Liebers, Fabien Chevalier, Silva Lerbs-Mache, Robert Blanvillain, Thomas Pfannschmidt
      Pages 253-271
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About this book

This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The chapters in this book also look at various techniques to analyze plastids through means of combining biology strategies from genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Plastids: Methods and Protocols is a valuable resource for students, engineers, and researchers who are interested in this evolving organelle and overall field.

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Keywords

  • plasticity
  • chloroplasts
  • Chromalveolata
  • Euglenozoa
  • endosymbionts
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Editors and Affiliations

  • Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l’Energie Atomique et aux Energies Alternatives, CEA Grenoble, Institut National Recherche Agronomique, UMR5168, Université Grenoble Alpes, Grenoble, France

    Eric Maréchal

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Bibliographic Information

  • Book Title: Plastids

  • Book Subtitle: Methods and Protocols

  • Editors: Eric Maréchal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8654-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8653-8Published: 10 July 2018

  • Softcover ISBN: 978-1-4939-9355-0Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8654-5Published: 09 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 409

  • Number of Illustrations: 20 b/w illustrations, 46 illustrations in colour

  • Topics: Plant Science

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Access via your institution
eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-8654-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 219.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)
Learn about institutional subscriptions