Skip to main content
  • Book
  • © 2018

Two-Hybrid Systems

Methods and Protocols

  • Includes cutting-edge methods and protocols for the study of two-hybrid systems
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1794)

Buy it now

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (24 protocols)

  1. Experimental Approaches to Study Protein-Protein and Protein-DNA Interactions in Plants and Human Cells

    1. KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein–Protein Interactions

      • Delphine Masschaele, Sarah Gerlo, Irma Lemmens, Sam Lievens, Jan Tavernier
      Pages 269-278
    2. Coimmunoprecipitation of Interacting Proteins in Plants

      • Alfonso Muñoz, M. Mar Castellano
      Pages 279-287
    3. Analysis of Protein–Protein Interaction by Co-IP in Human Cells

      • Zhenyuan Tang, Yoshinori Takahashi
      Pages 289-296
    4. Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures

      • Marta García-León, Elisa Iniesto, Vicente Rubio
      Pages 297-309
    5. Transient Transactivation Studies in Nicotiana benthamiana Leaves

      • Pilar Lasierra, Salomé Prat
      Pages 311-322
    6. Chromatin Immunoprecipitation for Identification of Protein–DNA Interactions in Human Cells

      • Bjørk Ditlev Larsen, Martin Rønn Madsen, Ronni Nielsen, Susanne Mandrup
      Pages 335-352
  2. Back Matter

    Pages 353-355

About this book

This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions.  The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include thekind of detail and key implementation advice that leads to excellent results in the lab. 

Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.

Editors and Affiliations

  • Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, Madrid, Spain

    Luis Oñate-Sánchez

Bibliographic Information

  • Book Title: Two-Hybrid Systems

  • Book Subtitle: Methods and Protocols

  • Editors: Luis Oñate-Sánchez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7871-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7870-0Published: 01 June 2018

  • Softcover ISBN: 978-1-4939-9310-9Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7871-7Published: 31 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 355

  • Number of Illustrations: 8 b/w illustrations, 50 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access