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  • © 2018

Planarian Regeneration

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1774)

Buying options

eBook USD 229.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7802-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 299.99
Price excludes VAT (USA)
Hardcover Book USD 399.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
  2. Planarian Diversity and Phylogeny

    • Ronald Sluys, Marta Riutort
    Pages 1-56
  3. The Ecology of Freshwater Planarians

    • Miquel Vila-Farré, Jochen C. Rink
    Pages 173-205
  4. Culturing Planarians in the Laboratory

    • M. Shane Merryman, Alejandro Sánchez Alvarado, James C. Jenkin
    Pages 241-258
  5. Total RNA Isolation from Planarian Tissues

    • Shang-Yun Liu, Jochen C. Rink
    Pages 259-265
  6. Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians

    • Markus A. Grohme, Miquel Vila-Farré, Jochen C. Rink
    Pages 267-275
  7. Planarian High Molecular Weight DNA Isolation by Spooling

    • Shasha Zhang, Alejandro Sánchez Alvarado
    Pages 277-284
  8. Basic Histological Techniques for Planarians

    • Leigh Winsor, Ronald Sluys
    Pages 285-351
  9. Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians

    • David J. Forsthoefel, Kelly G. Ross, Phillip A. Newmark, Ricardo M. Zayas
    Pages 353-366
  10. Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections

    • Teresa Adell, Sara Barberán, Miquel Sureda-Gómez, María Almuedo-Castillo, Nidia de Sousa, Francesc Cebrià
    Pages 367-378
  11. Whole-Mount In Situ Hybridization of Planarians

    • Ryan S. King, Phillip A. Newmark
    Pages 379-392
  12. Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians

    • Li-Chun Cheng, Alejandro Sánchez Alvarado
    Pages 423-434
  13. Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL

    • Brad Stubenhaus, Jason Pellettieri
    Pages 435-444
  14. Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria

    • Carolyn E. Adler, Alejandro Sánchez Alvarado
    Pages 445-454

About this book

This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Comprehensive and cutting-edge, Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.

Keywords

  • pluripotent adult stem cells
  • planarian ecology
  • RNA extraction
  • DNA extraction
  • single stem cell transplantations
  • electron microscopy
  • metabolomics analysis
  • model organisms

Editors and Affiliations

  • Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

    Jochen C. Rink

About the editor

Jochen Rink, PhD
Group Leader
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden, Germany
Tel: +49-(0)351-210-2435
Email: rink@mpi-cbg.de 

Bibliographic Information

  • Book Title: Planarian Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Jochen C. Rink

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7802-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7800-7

  • Softcover ISBN: 978-1-4939-9289-8

  • eBook ISBN: 978-1-4939-7802-1

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 573

  • Number of Illustrations: 36 b/w illustrations, 56 illustrations in colour

  • Topics: Cell Biology

Buying options

eBook USD 229.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7802-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 299.99
Price excludes VAT (USA)
Hardcover Book USD 399.99
Price excludes VAT (USA)