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  • © 2018

Chromothripsis

Methods and Protocols

Editors:

  1. Franck Pellestor

    1. Unit of Chromosomal Genetics, Department of Medical Genetics, CHU Montpellier, Montpellier, France

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  • Includes cutting-edge methods and protocols for the study of chromothripsis

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1769)

Sections

eBook USD 169.00
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  • ISBN: 978-1-4939-7780-2
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Softcover Book USD 219.99
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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    PDF

  2. Genesis and Impact of Chromothripsis

    1. Front Matter

      Pages 1-1
      PDF

    2. The Genomic Characteristics and Origin of Chromothripsis

      • Alessio Marcozzi, Franck Pellestor, Wigard P. Kloosterman
      Pages 3-19

    5. Chromothripsis and the Macroevolution Theory

      • Franck Pellestor
      Pages 43-49

  3. The Identification of Chromothripsis

    1. Front Matter

      Pages 51-51
      PDF

    4. Identification of Chromothripsis in Biopsy Using SNP-Based Microarray

      • Veronica Ortega, Christina Mendiola, Gopalrao V. N. Velagaleti
      Pages 85-117

    5. Detection of Chromothripsis in Plants

      • Isabelle M. Henry, Luca Comai, Ek Han Tan
      Pages 119-132

    6. RNA-Seq Analysis to Detect Abnormal Fusion Transcripts Linked to Chromothripsis

      • Anne-Laure Bougé, Florence Rufflé, Sébastien Riquier, Benoit Guibert, Jérôme Audoux, Thérèse Commes
      Pages 133-156

  4. The Causal Mechanisms of Chromothripsis

    1. Front Matter

      Pages 157-157
      PDF

    3. A Role for Retrotransposons in Chromothripsis

      • Dustin C. Hancks
      Pages 169-181

    4. Generation of Micronuclei and Detection of Chromosome Pulverization

      • Monique N. H. Luijten, Jeannie X. T. Lee, Sixun Chen, Karen C. Crasta
      Pages 183-195

    6. Study of Telomere Dysfunction in TP53 Mutant LoVo Cell Lines as a Model for Genomic Instability

      • Oumar Samassekou, Nathalie Bastien, Ju Yan, Sabine Mai, Régen Drouin
      Pages 209-230

    8. Expression of Genes Associated with Telomere Homeostasis in TP53 Mutant LoVo Cell Lines as a Model for Genomic Instability

      • Oumar Samassekou, Nathalie Bastien, Ju Yan, Sabine Mai, Régen Drouin
      Pages 253-262
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About this book

This detailed book presents an up-to-date view on methods and experimental approaches developed to identify and explore the chromothripsis phenomenon. Beginning with a section exploring the genesis and impact of chromothripsis, the collection continues by covering the identification of chromothripsis, the causal mechanisms of chromothripsis, the bioinformatics tools for chromothripsis analysis, and experimental systems recently developed for the in vitro investigation of chromothripsis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Chromothripsis: Methods and Protocols serves as a vital resource for cell biologists, molecular biologists, cytogeneticists, and geneticists investigating chromothripsis, but also for students and researchers new to the field of chromothripsis and genomic instability.
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Keywords

  • Massive genomic alterations
  • DNA fragments
  • Complex derivative chromosomes
  • Cytogenetics
  • Bioinformatics
  • Cancer research
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Editors and Affiliations

  • Unit of Chromosomal Genetics, Department of Medical Genetics, CHU Montpellier, Montpellier, France

    Franck Pellestor

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Bibliographic Information

  • Book Title: Chromothripsis

  • Book Subtitle: Methods and Protocols

  • Editors: Franck Pellestor

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7780-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7779-6Published: 21 March 2018

  • Softcover ISBN: 978-1-4939-9283-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7780-2Published: 21 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 367

  • Number of Illustrations: 12 b/w illustrations, 73 illustrations in colour

  • Topics: Medical Genetics

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Access via your institution
eBook USD 169.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7780-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 219.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)
Learn about institutional subscriptions