Host-Pathogen Interactions

1. Front Matter

   Pages i-xvi

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2. Genetic Association Studies in Host–Pathogen Interaction Analysis

   • Jose Luis Royo, Luis Miguel Real

   Pages 1-11

3. Bacterial Genotyping Methods: From the Basics to Modern

   • Margarita M. Ochoa-Díaz, Silvana Daza-Giovannetty, Doris Gómez-Camargo

   Pages 13-20

4. Animal-Pathogen Interaction Section

   1. Front Matter

      Pages 21-21

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   2. Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

      • Gili Aviv, Ohad Gal-Mor

      Pages 23-32

   3. Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection

      • Gili Aviv, Ohad Gal-Mor

      Pages 33-38

   4. lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

      • Gili Aviv, Ohad Gal-Mor

      Pages 39-45

   5. Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity

      • Gili Aviv, Ohad Gal-Mor

      Pages 47-54

   6. Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage

      • Carlos Medina, Beatriz Mesa-Pereira, Eva M. Camacho, Amando Flores, Eduardo Santero

      Pages 55-70

   7. Organoids as a Model to Study Infectious Disease

      • Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, Eitaro Aihara

      Pages 71-81

   8. Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins

      • Anita Horvatić, Josipa Kuleš, Nicolas Guillemin, Franjo Martinković, Iva Štimac, Vladimir Mrljak et al.

      Pages 83-96

   9. Systems Biology Modeling to Study Pathogen–Host Interactions

      • Müberra Fatma Cesur, Saliha Durmuş

      Pages 97-112

   10. Phage Therapy: Various Perspectives on How to Improve the Art

       • Stephen T. Abedon

       Pages 113-127

5. Microbe-Microbe Interaction Section

   1. Front Matter

      Pages 129-129

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   2. Application of RNA-seq and Bioimaging Methods to Study Microbe–Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression

      • Cristina Isabel Amador, Claus Sternberg, Lars Jelsbak

      Pages 131-158

   3. Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth

      • Fernando Govantes

      Pages 159-169

   4. Detection of Bacterial Quorum Sensing Molecules

      • Elke Stein, Adam Schikora

      Pages 171-179

6. Plant-Pathogen Interaction Section

   1. Front Matter

      Pages 181-181

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   2. Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae

      • José S. Rufián, Diego López-Márquez, Nieves López-Pagán, Murray Grant, Javier Ruiz-Albert, Carmen R. Beuzón

      Pages 183-199

   3. Introduction of Genetic Material in Ralstonia solanacearum Through Natural Transformation and Conjugation

      • Anthony Perrier, Patrick Barberis, Stéphane Genin

      Pages 201-207

This detailed volume explores numerous multidisciplinary approaches employed to analyze the role of different molecules or strategies used by different guests to survive inside hosts. Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Host-Pathogen Interactions: Methods and Protocols provides many molecular and bioinformatics techniques currently in use at reputed laboratories in countries all around the world, vital for many kinds of host-pathogen interactions studies.

• Microbes
• Microorganisms
• Animal hosts
• Salmonella
• Bacterium
• Plant hosts
• Bioinformatic techniques

• Departamento de Biología Molecular e Ingeniería Bioquímica, Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de, Olavide/Junta de Andalucía, Seville, Spain

  Carlos Medina

• Department of Microbiology, University of Seville, Sevilla, Spain

  Francisco Javier López-Baena

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