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Peptidomics

Methods and Strategies

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible products
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1719)

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Table of contents (28 protocols)

  1. Diverse Applied Protocols and Outlook

    1. Identification, Quantitation, and Imaging of the Crustacean Peptidome

      • Kellen DeLaney, Amanda Buchberger, Lingjun Li
      Pages 247-269
    2. Identification of Endogenous Neuropeptides in the Nematode C. elegans Using Mass Spectrometry

      • Sven Van Bael, Samantha L. Edwards, Steven J. Husson, Liesbet Temmerman
      Pages 271-291
    3. EndoProteoFASP as a Tool to Unveil the Peptidome-Protease Profile: Application to Salivary Diagnostics

      • Fábio Trindade, Inês Falcão-Pires, Adelino Leite-Moreira, Pedro S. Gomes, Julie Klein, Rita Ferreira et al.
      Pages 293-310
    4. Methodology for Urine Peptidome Analysis Based on Nano-HPLC Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

      • Alexey S. Kononikhin, Victoria A. Sergeeva, Anna E. Bugrova, Maria I. Indeykina, Natalia L. Starodubtseva, Vitaliy V. Chagovets et al.
      Pages 311-318
    5. Identification of Components in Frog Skin Secretions with Therapeutic Potential as Antidiabetic Agents

      • J. Michael Conlon, R. Charlotte Moffett, Jérôme Leprince, Peter R. Flatt
      Pages 319-333
    6. High-Accuracy Mass Spectrometry Based Screening Method for the Discovery of Cysteine Containing Peptides in Animal Venoms and Toxins

      • Luuk N. van Oosten, Martijn W. H. Pinkse, Mervin Pieterse, Pierre Escoubas, Peter D. E. M. Verhaert
      Pages 335-348
    7. Analysis of the Snake Venom Peptidome

      • Solange M. T. Serrano, André Zelanis, Eduardo S. Kitano, Alexandre K. Tashima
      Pages 349-358
    8. Identification of Peptides in Spider Venom Using Mass Spectrometry

      • Rafael L. Lomazi, Erika S. Nishiduka, Pedro I. Silva Jr, Alexandre K. Tashima
      Pages 359-367
    9. Peptidomic Identification of Cysteine-Rich Peptides from Plants

      • Xinya Hemu, Aida Serra, Dina A. Darwis, Tobias Cornvik, Siu Kwan Sze, James P. Tam
      Pages 379-393
    10. Analysis of Endogenous Peptide Pools of Physcomitrella patens Moss

      • Igor Fesenko, Regina Khazigaleeva, Vadim Govorun, Vadim Ivanov
      Pages 395-405
    11. The Bright Future of Peptidomics

      • Peter D. E. M. Verhaert
      Pages 407-416
  2. Back Matter

    Pages 417-423

About this book

This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls.

Authoritative and practical, Peptidomics: Methods and Strategies provides useful guidance for studies in the rapidly growing field of peptidomics. 

Editors and Affiliations

  • Department of Biotechnology and Bioinformatics, Weihenstephan-Tr. University of Applied Sciences, Freising, Germany

    Michael Schrader

  • Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, USA

    Lloyd Fricker

Bibliographic Information

  • Book Title: Peptidomics

  • Book Subtitle: Methods and Strategies

  • Editors: Michael Schrader, Lloyd Fricker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7537-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7536-5Published: 24 February 2018

  • Softcover ISBN: 978-1-4939-8514-2Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7537-2Published: 23 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 423

  • Number of Illustrations: 62 b/w illustrations, 17 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access