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  • © 2018

Bacterial Multidrug Exporters

Methods and Protocols

  • Includes cutting-edge methods and protocols for the study of bacterial multidrug exporters
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1700)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
  2. Structural Analysis of Bacterial Multidrug Exporters

    1. Front Matter

      Pages 1-1
    2. Crystallographic Analysis of MATE-Type Multidrug Exporter with Its Inhibitors

      • Tsukasa Kusakizako, Yoshiki Tanaka, Christopher J. Hipolito, Hiroaki Suga, Osamu Nureki
      Pages 37-57
    3. Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis

      • Dijun Du, Zhao Wang, Wah Chiu, Ben F. Luisi
      Pages 71-81
    4. Generation of Conformation-Specific Antibody Fragments for Crystallization of the Multidrug Resistance Transporter MdfA

      • Frank Jaenecke, Yoshiko Nakada-Nakura, Kumar Nagarathinam, Satoshi Ogasawara, Kehong Liu, Yunhon Hotta et al.
      Pages 97-109
  3. Biochemical and Bioengineering Analysis of Bacterial Multidrug Exporters

    1. Front Matter

      Pages 111-111
    2. Biochemical Reconstitution and Characterization of Multicomponent Drug Efflux Transporters

      • Martin Picard, Elena B. Tikhonova, Isabelle Broutin, Shuo Lu, Alice Verchère, Helen I. Zgurskaya
      Pages 113-145
  4. Computational Analysis of Bacterial Multidrug Exporters

    1. Front Matter

      Pages 177-177
    2. Molecular Modeling of Multidrug Properties of Resistance Nodulation Division (RND) Transporters

      • Pierpaolo Cacciotto, Venkata K. Ramaswamy, Giuliano Malloci, Paolo Ruggerone, Attilio V. Vargiu
      Pages 179-219
    3. A Transcriptomic Approach to Identify Novel Drug Efflux Pumps in Bacteria

      • Liping Li, Sasha G. Tetu, Ian T. Paulsen, Karl A. Hassan
      Pages 221-235
  5. Biomedical Approach for Bacterial Multidrug Exporters

    1. Front Matter

      Pages 237-237
    2. Study of the Expression of Bacterial Multidrug Efflux Pumps in Anaerobic Conditions

      • Jingjing Sun, Ziqing Deng, Danny Ka Chun Fung, Aixin Yan
      Pages 253-268

About this book

This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria. Beginning with protocols for the structural analysis of bacterial multidrug exporters, the book continues with sections on biochemical and bioengineering analysis, computational analysis, biomedical approaches, as well as advanced technologies expected to be applied to multidrug efflux transport studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bacterial Multidrug Exporters: Methods and Protocols serves as an ideal guide to

this fastmoving field of study.

Editors and Affiliations

  • Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Japan

    Akihito Yamaguchi

  • Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan

    Kunihiko Nishino

Bibliographic Information

  • Book Title: Bacterial Multidrug Exporters

  • Book Subtitle: Methods and Protocols

  • Editors: Akihito Yamaguchi, Kunihiko Nishino

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7454-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7452-8Published: 25 November 2017

  • Softcover ISBN: 978-1-4939-8490-9Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7454-2Published: 23 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 355

  • Number of Illustrations: 38 b/w illustrations, 53 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access