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  • © 2018

Borrelia burgdorferi

Methods and Protocols

Editors:

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  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1690)

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eBook
USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7383-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
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  • Tax calculation will be finalised during checkout
Softcover Book
USD 159.00
Price excludes VAT (USA)
Hardcover Book
USD 169.99
Price excludes VAT (USA)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
  2. Analysis of Lipids and Lipid Rafts in Borrelia

    • Alvaro Toledo, Zhen Huang, Jorge L. Benach, Erwin London
    Pages 69-82
  3. Borrelia burgdorferi Microarray Analysis

    • Radha Iyer, Ira Schwartz
    Pages 115-126
  4. Analysis of DNA and RNA Binding Properties of Borrelia burgdorferi Regulatory Proteins

    • J. Seshu, Trever C. Smith II, Ying-Han Lin, S. L. Rajasekhar Karna, Christine L. Miller, Tricia Van Laar
    Pages 155-175
  5. Identification of Acetylated Proteins in Borrelia burgdorferi

    • Youyun Yang, Alan Wolfe, X. Frank Yang
    Pages 177-182
  6. Genetic Transformation and Complementation

    • D. Scott Samuels, Dan Drecktrah, Laura S. Hall
    Pages 183-200
  7. Genome-Wide Mutagenesis in Borrelia burgdorferi

    • Tao Lin, Lihui Gao
    Pages 201-223
  8. Generation of Conditional Mutants in Borrelia burgdorferi

    • Jacob I. Latham, Jon S. Blevins
    Pages 225-239
  9. Analysis of Borrelia burgdorferi Proteome and Protein–Protein Interactions

    • Xiuli Yang, Meghna Thakur, Juraj Koci, Alexis A. Smith, Preeti Singh, Xuran Zhuang et al.
    Pages 259-277

About this book

This volume details protocols that broadly cover many aspects of basic and translational research on Borrelia burgdorferi. Chapters guide readers through epidemiology and ecology, cultivation, cell structure, physiology, genomics and transcriptomics, proteomics, animal infection, pathogenesis and host responses, and vaccines. These essential protocols incorporate the most recent, practical, and innovative research tools  aiding new and experienced researchers in their studies involving the biology, pathogenesis, and prevention of B. burgdorferi infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Borrelia burgdorferi: Methods and Protocols aims to ensure successful results in the further study of this vital field of biomedical research.

Keywords

  • bacterial pathogen
  • Lyme disease
  • epidemiology
  • ecology
  • vector
  • infectious diseases

Editors and Affiliations

  • Department of Veterinary Medicine, University of Maryland, College Park, USA

    Utpal Pal

  • Department of Microbiology, Ondokuz Mayis University, Samsun, Turkey

    Ozlem Buyuktanir

Bibliographic Information

  • Book Title: Borrelia burgdorferi

  • Book Subtitle: Methods and Protocols

  • Editors: Utpal Pal, Ozlem Buyuktanir

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7383-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7382-8

  • Softcover ISBN: 978-1-4939-8468-8

  • eBook ISBN: 978-1-4939-7383-5

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 361

  • Number of Illustrations: 21 b/w illustrations, 31 illustrations in colour

  • Topics: Diseases

Buying options

eBook
USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7383-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 159.00
Price excludes VAT (USA)
Hardcover Book
USD 169.99
Price excludes VAT (USA)