Cellular Quiescence

Methods and Protocols

  • H. Daniel Lacorazza

Part of the Methods in Molecular Biology book series (MIMB, volume 1686)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mithun Mitra, Linda D. Ho, Hilary A. Coller
    Pages 27-47
  3. Ayad Eddaoudi, Stephanie Louise Canning, Itaru Kato
    Pages 49-57
  4. Alexandra Chicheportiche, Martial Ruat, François D. Boussin, Mathieu Daynac
    Pages 69-78
  5. Katie A. Matatall, Claudine S. Kadmon, Katherine Y. King
    Pages 91-103
  6. Gregory K. Behbehani
    Pages 105-124
  7. Marla M. Spain, Sarah G. Swygert, Toshio Tsukiyama
    Pages 125-135
  8. Christine N. Rodriguez, Hoang Nguyen
    Pages 137-147
  9. Caroline E. Brun, Yu Xin Wang, Michael A. Rudnicki
    Pages 149-159
  10. Chun Shik Park, H. Daniel Lacorazza
    Pages 173-182
  11. Surabhi Srivastava, Hardik P. Gala, Rakesh K. Mishra, Jyotsna Dhawan
    Pages 215-239
  12. Syed I. A. Bukhari, Samuel S. Truesdell, Shobha Vasudevan
    Pages 251-264

About this book

Introduction

This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology.

Keywords

Cellular dormancy Stem cells Prokaryotes Eukaryotes Genomic regulation

Editors and affiliations

  • H. Daniel Lacorazza
    • 1
  1. 1.Texas Children’s HospitalBaylor College of MedicineHoustonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7371-2
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7370-5
  • Online ISBN 978-1-4939-7371-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book