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  • © 2018

Potassium Channels

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1684)

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7362-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.00
Price excludes VAT (USA)
Hardcover Book USD 149.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (25 protocols)

  1. Front Matter

    Pages i-xvi
  2. Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions

    • Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, Ichio Shimada
    Pages 115-128
  3. Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes

    • Josefina del Mármol, Robert A. Rietmeijer, Stephen G. Brohawn
    Pages 129-150
  4. Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET

    • Shizhen Wang, Joshua B. Brettmann, Colin G. Nichols
    Pages 163-180
  5. Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters

    • Kimberly Matulef, Francis I. Valiyaveetil
    Pages 181-191
  6. Combinatorial Assembly of Lumitoxins

    • David Nedrud, Daniel Schmidt
    Pages 193-209
  7. Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay

    • Michael J. Litt, Roger D. Cone, Masoud Ghamari-Langroudi
    Pages 211-222
  8. Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies

    • David J. Posson, Radda Rusinova, Olaf S. Andersen, Crina M. Nimigean
    Pages 223-235

About this book

This volume provides readers with the latest techniques and approaches used to study K+ channels. There are a wide range of topics covered in the book, such as channel structure; K+ channels and their roles in physiology and disease; single-molecule FRET; proteins expression levels in vivo; real-time correlation of channel activity; gating; pharmacology; physiological functions; and channelopathies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Potassium Channels: Methods and Protocols is a valuable resource for all researchers who are interested in studying potassium channels.

Keywords

  • membrane excitability
  • ion channels
  • channel structure
  • channelopathies
  • Patching K+ channels
  • pharmacology

Editors and Affiliations

  • Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, USA

    Show-Ling Shyng, Francis I. Valiyaveetil

  • The Vollum Institute, Oregon Health and Science University, Portland, USA

    Matt Whorton

Bibliographic Information

  • Book Title: Potassium Channels

  • Book Subtitle: Methods and Protocols

  • Editors: Show-Ling Shyng, Francis I. Valiyaveetil, Matt Whorton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7362-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7361-3

  • Softcover ISBN: 978-1-4939-8462-6

  • eBook ISBN: 978-1-4939-7362-0

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 345

  • Number of Illustrations: 31 b/w illustrations, 53 illustrations in colour

  • Topics: Biochemistry

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7362-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.00
Price excludes VAT (USA)
Hardcover Book USD 149.99
Price excludes VAT (USA)