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  • © 2017

Super-Resolution Microscopy

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1663)

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eBook USD 129.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7265-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 169.99
Price excludes VAT (USA)
Hardcover Book USD 169.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics

    • Jan Philipp Eberle, Alexander Rapp, Matthias Krufczik, Marion Eryilmaz, Manuel Gunkel, Holger Erfle et al.
    Pages 1-13
  3. Live-Cell STED Imaging with the HyPer2 Biosensor

    • Natalia M. Mishina, Vsevolod V. Belousov
    Pages 21-28
  4. Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo

    • Mirelle J. T. ter Veer, Thomas Pfeiffer, U. Valentin Nägerl
    Pages 45-64
  5. Correlative SIM-STORM Microscopy

    • O. Burri, T. Laroche, R. Guiet, A. Seitz
    Pages 95-103
  6. Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles

    • Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann
    Pages 115-126
  7. Two-Color Single-Molecule Tracking in Live Cells

    • Siegfried Hänselmann, Dirk-Peter Herten
    Pages 127-138
  8. Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy

    • Jan Philipp Eberle, Walter Muranyi, Holger Erfle, Manuel Gunkel
    Pages 139-152
  9. Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy

    • Christopher L. German, Manasa V. Gudheti, Annette E. Fleckenstein, Erik M. Jorgensen
    Pages 153-162
  10. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments

    • Jonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothée Lionnet, Luke D. Lavis
    Pages 179-188
  11. Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step

    • Susanna Maria Früh, Ingmar Schoen
    Pages 189-203
  12. Correlative Single-Molecule Localization Microscopy and Confocal Microscopy

    • Christian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, David Crossman
    Pages 205-217
  13. In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT

    • Brian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang et al.
    Pages 231-252

About this book

This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

Keywords

  • Stimulated Emission Depletion
  • Ground State Depletion
  • Photo Activated Localization
  • fluorescence
  • STORM

Reviews

“The aim is to provide a current overview of the advanced technologies and methods in super‐resolution microscopy. The book is written for novices and experienced investigators. It will be of much help for graduate students and postdoctoral students interested in super-resolution microscopy. The author is a credible authority in the field of super-resolution microscopy. … This book covers advanced, cutting-edge techniques related to super-resolution microscopy. The intended audience will find it of immense help.” (Omer Iqbal, Doody's Book Reviews, February, 2018)

Editors and Affiliations

  • High-Content Analysis of the Cell (HiCell) and Advanced Biological Screening Facility, BioQuant, Heidelberg University, Heidelberg, Germany

    Holger Erfle

Bibliographic Information

  • Book Title: Super-Resolution Microscopy

  • Book Subtitle: Methods and Protocols

  • Editors: Holger Erfle

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7265-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7264-7

  • Softcover ISBN: 978-1-4939-8435-0

  • eBook ISBN: 978-1-4939-7265-4

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 262

  • Number of Illustrations: 11 b/w illustrations, 37 illustrations in colour

  • Topics: Radiology

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7265-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 169.99
Price excludes VAT (USA)
Hardcover Book USD 169.99
Price excludes VAT (USA)