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Plant Protein Secretion

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Liwen Jiang

    1. School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1662)

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About this book

This volume aims to provide an update on recent developments in protein secretion studies in plants versus yeast and mammalian systems. This book also discusses case studies that analyze the use of plant protein secretion using various tools and systems. The chapters in this book explore topics such as the study of Golgi-mediated protein traffic in plant cells; actin-based intracellular trafficking in pollen tubes; secretion system for identification of cargo proteins of vacuolar sorting receptors; isolation of the plant exocyst complex; and plant autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Plant Protein Secretion: Methods and Protocols is a valuable resource for researchers interested in furthering their studies in plant protein secretion."

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. An Overview of Protein Secretion in Yeast and Animal Cells

    • Yusong Guo, Feng Yang, Xiao Tang
    Pages 1-17

  3. An Overview of Protein Secretion in Plant Cells

    • Kin Pan Chung, Yonglun Zeng
    Pages 19-32

  4. Bioinformatics Analysis of Protein Secretion in Plants

    • Liyuan Chen
    Pages 33-43

  5. Proteomic Analysis of Secreted Proteins from Cell Microenvironment

    • Subash Adhikari, Lan Chen, Peiwu Huang, Ruijun Tian
    Pages 45-58

  6. Using Homology Modeling to Understand the Structural Basis of Specific Interaction of a Plant-Specific AtSar1a–AtSec23a Pair Involved in Protein ER Export

    • Yap-Shing Nim, Shuangli Sun, Kam-Bo Wong
    Pages 59-73

  7. Analysis of Golgi-Mediated Protein Traffic in Plant Cells

    • Wenjin Shen, Zhidan Xiao, Jinbo Shen, Caiji Gao
    Pages 75-86

  8. Analysis of Membrane Protein Topology in the Plant Secretory Pathway

    • Jinya Guo, Yansong Miao, Yi Cai
    Pages 87-95

  9. Semiautomatic Segmentation of Plant Golgi Stacks in Electron Tomograms Using 3dmod

    • Keith Ka Ki Mai, Byung-Ho Kang
    Pages 97-104

  10. 3D Printing of Plant Golgi Stacks from Their Electron Tomographic Models

    • Keith Ka Ki Mai, Madison J. Kang, Byung-Ho Kang
    Pages 105-113

  11. Transient Expression of Chimeric Fluorescent Reporter Proteins in Pollen Tubes to Study Protein Polar Secretion and Dynamics

    • Guitao Zhong, Ronghe Liu, Menglong Zhuang, Hao Wang
    Pages 115-124

  12. Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes

    • Yuxiang Jiang, Meng Zhang, Shanjin Huang
    Pages 125-136

  13. Analysis of Phragmoplast Kinetics During Plant Cytokinesis

    • Pantelis Livanos, Mayank Chugh, Sabine Müller
    Pages 137-150

  14. Immunofluorescence Analysis of Membrane-Associated Proteins for Clathrin-Mediated Endocytosis in Plant Root Cells

    • Chao Wang, Xu Yan, Tingting Meng, Tianwei Hu, Jianwei Pan
    Pages 151-157

  15. In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer Through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM)

    • Florian Fäßler, Peter Pimpl
    Pages 159-170

  16. Analysis of Nanobody–Epitope Interactions in Living Cells via Quantitative Protein Transport Assays

    • Simone Früholz, Peter Pimpl
    Pages 171-182

  17. A Secretion System for Cargo Protein Identification of Vacuolar Sorting Receptors

    • Jinbo Shen
    Pages 183-191

  18. Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination

    • Qiang-Nan Feng, Yan Zhang
    Pages 193-198

  19. Measuring Plant Protein Secretion

    • Emily R. Larson
    Pages 199-207

  20. Transient Secretory Enzyme Expression in Leaf Protoplasts to Characterize SNARE Functional Classes in Conventional and Unconventional Secretion

    • Di Sansebastiano Gian Pietro, Barozzi Fabrizio
    Pages 209-221
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Editors and Affiliations

  • School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China

    Liwen Jiang

Bibliographic Information

  • Book Title: Plant Protein Secretion

  • Book Subtitle: Methods and Protocols

  • Editors: Liwen Jiang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7262-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7261-6Published: 02 September 2017

  • Softcover ISBN: 978-1-4939-8434-3Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7262-3Published: 31 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 285

  • Number of Illustrations: 8 b/w illustrations, 60 illustrations in colour

  • Topics: Plant Sciences

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