c-di-GMP Signaling

Methods and Protocols

  • Karin Sauer

Part of the Methods in Molecular Biology book series (MIMB, volume 1657)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Ute Römling, Michael Y. Galperin
    Pages 1-8
  3. Synthesis of c-di-GMP

  4. Detection and Quantitation of c-di-GMP

  5. Visualizing c-di-GMP Levels Using Biosensors

    1. Front Matter
      Pages 85-85
    2. Morten Rybtke, Song Lin Chua, Joey Kuok Hoong Yam, Michael Givskov, Liang Yang, Tim Tolker-Nielsen
      Pages 87-98
    3. Bradley R. Borlee, Grace I. Borlee, Kevin H. Martin, Yasuhiko Irie
      Pages 99-110
    4. Jongchan Yeo, Xin C. Wang, Ming C. Hammond
      Pages 111-130
  6. Indirect Detection of c-di-GMP Levels

  7. Modulation of c-di-GMP Levels and Bacterial Responses

    1. Front Matter
      Pages 167-167
    2. Min-Hyung Ryu, Anastasia Fomicheva, Lindsey O’Neal, Gladys Alexandre, Mark Gomelsky
      Pages 169-186

About this book


This volume provides a collection of protocols for the common experimental approaches used in the in the burgeoning field of c-di-GMP-dependent signaling. The chapters, divided into eight major parts, guide readers through methods on synthesis, detection, quantitation, modulation of the levels of c-di-GMP present in cells, procedures to detect and evaluate the interaction of c-di-GMP, and up and coming approaches focusing on the inhibition of c-di-GMP signaling.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, c-di-GMP Signaling: Methods and Protocols aims to inspire researchers to try new approaches.


Pseudomonas aeruginosa FimA HPLC c-di-GMP signaling Signal transduction

Editors and affiliations

  • Karin Sauer
    • 1
  1. 1.Department of Biological SciencesBinghamton University Department of Biological SciencesBinghamtonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7240-1
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7239-5
  • Online ISBN 978-1-4939-7240-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book