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RNA Detection

Methods and Protocols

  • Book
  • © 2018

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1649)

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About this book

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

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Keywords

Table of contents (32 protocols)

Editors and Affiliations

  • Developmental Biology Unit, EMBL Heidelberg, Heidelberg, Germany

    Imre Gaspar

Bibliographic Information

  • Book Title: RNA Detection

  • Book Subtitle: Methods and Protocols

  • Editors: Imre Gaspar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7213-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7212-8Published: 15 November 2017

  • Softcover ISBN: 978-1-4939-8418-3Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7213-5Published: 11 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 492

  • Number of Illustrations: 16 b/w illustrations, 78 illustrations in colour

  • Topics: Human Genetics

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