Book Volume 1648 2017

mRNA Processing

Methods and Protocols

Editors:

ISBN: 978-1-4939-7203-6 (Print) 978-1-4939-7204-3 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Protocol

    Pages 1-9

    Detection and Identification of Uncapped RNA by Ligation-Mediated Reverse Transcription Polymerase Chain Reaction

  3. No Access

    Protocol

    Pages 11-26

    Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay

  4. No Access

    Protocol

    Pages 27-37

    Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS)

  5. No Access

    Protocol

    Pages 39-52

    In Vitro Modulation of Endogenous Alternative Splicing Using Splice-Switching Antisense Oligonucleotides

  6. No Access

    Protocol

    Pages 53-63

    Purification of mRNA Processing Complexes Using an RNA Affinity Approach

  7. No Access

    Protocol

    Pages 65-77

    Polyadenylation Site-Based Analysis of Transcript Expression by 3′READS+

  8. No Access

    Protocol

    Pages 79-93

    Comprehensive Identification of mRNA Polyadenylation Sites by PAPERCLIP

  9. No Access

    Protocol

    Pages 95-102

    Microinjection and Fluorescence In Situ Hybridization Assay for Studying mRNA Export in Mammalian Cells

  10. No Access

    Protocol

    Pages 103-127

    Methods for the Detection of Adenosine-to-Inosine Editing Events in Cellular RNA

  11. No Access

    Protocol

    Pages 129-142

    Using RNA-Seq to Discover Genetic Polymorphisms That Produce Hidden Splice Variants

  12. No Access

    Protocol

    Pages 143-154

    Inducible Expression of Eukaryotic Circular RNAs from Plasmids

  13. No Access

    Protocol

    Pages 155-167

    Methods for Extraction of RNA, Proteins, or Protein Complexes from Subcellular Compartments of Eukaryotic Cells

  14. No Access

    Protocol

    Pages 169-176

    Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine

  15. No Access

    Protocol

    Pages 177-200

    Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)

  16. No Access

    Protocol

    Pages 201-220

    Purification of Transcript-Specific mRNP Complexes Formed In Vivo from Saccharomyces cerevisiae

  17. No Access

    Protocol

    Pages 221-233

    A Cell-Based High-Throughput Method for Identifying Modulators of Alternative Splicing

  18. No Access

    Protocol

    Pages 235-245

    Genome-Wide RNAi Screens for RNA Processing Events in Drosophila melanogaster S2 Cells

  19. No Access

    Protocol

    Pages 247-256

    Assaying RNA Structure Inside Living Cells with SHAPE

  20. Back Matter

    Pages 257-258