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Fast Detection of DNA Damage

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Vladimir V. Didenko

    1. Department of Neurosurgery and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1644)

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About this book

This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.

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Keywords

Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Express Detection (from 3 min to 1 h)

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Express FRET Labeling and Analysis of Phagocytic Clearance

      • Vladimir V. Didenko
      Pages 3-11

    3. Rapid Assessment of Genotoxicity by Flow Cytometric Detection of Cell Cycle Alterations

      • Nevenka Bihari
      Pages 13-21

    4. Ultrasound Imaging of DNA-Damage Effects in Live Cultured Cells and in Brain Tissue

      • Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, Gregory J. Czarnota
      Pages 23-40

    5. Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects In Vivo

      • Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, Gregory J. Czarnota
      Pages 41-60

    6. Fluorochrome-Labeled Inhibitors of Caspases: Expedient In Vitro and In Vivo Markers of Apoptotic Cells for Rapid Cytometric Analysis

      • Zbigniew Darzynkiewicz, Hong Zhao, H. Dorota Halicka, Piotr Pozarowski, Brian Lee
      Pages 61-73

    7. The Fast-Halo Assay for the Detection of DNA Damage

      • Piero Sestili, Cinzia Calcabrini, Anna Rita Diaz, Carmela Fimognari, Vilberto Stocchi
      Pages 75-93

    8. Rapid Detection of Bacterial Susceptibility or Resistance to Quinolones

      • Fátima Otero, Rebeca Santiso, María Tamayo, Germán Bou, Jaime Gosálvez, José Luis Fernández
      Pages 95-104

    9. Rapid Detection of Apoptosis in Cultured Mammalian Cells

      • Igor Kudryavtsev, Maria Serebryakova, Liudmila Solovjeva, Maria Svetlova, Denis Firsanov
      Pages 105-111

    10. Quick Detection of DNase II-Type Breaks in Formalin-Fixed Tissue Sections

      • Candace L. Minchew, Vladimir V. Didenko
      Pages 113-119

  3. Rapid Detection (2–3 h)

    1. Front Matter

      Pages 121-121
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    2. Express γ-H2AX Immunocytochemical Detection of DNA Damage

      • Nate Hopp, Jodi Hagen, Birte Aggeler, Alexander E. Kalyuzhny
      Pages 123-128

    3. Rapid Detection of γ-H2AX by Flow Cytometry in Cultured Mammalian Cells

      • Denis Firsanov, Liudmila Solovjeva, Olga Lublinskaya, Valeriy Zenin, Igor Kudryavtsev, Maria Serebryakova et al.
      Pages 129-138

    4. Rapid Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry

      • Hong Zhao, Zbigniew Darzynkiewicz
      Pages 139-149

    5. Fast Micromethod: Determination of DNA Integrity in Cell Suspensions and in Solid Tissues

      • Nevenka Bihari
      Pages 151-158

    6. Quantification of DNA Damage and Repair in Mitochondrial, Nuclear, and Bacterial Genomes by Real-Time PCR

      • Nadja Patenge
      Pages 159-166

    7. Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt-Ended DNA Breaks by In Situ Ligation

      • Vladimir V. Didenko
      Pages 167-177

  4. Accelerated Detection (Time-Saving Versions of Conventional Techniques)

    1. Front Matter

      Pages 179-179
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    2. Twelve-Gel Comet Assay Format for Quick Examination of DNA Damage and Repair

      • Sergey Shaposhnikov, Andrew Collins
      Pages 181-186
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Editors and Affiliations

  • Department of Neurosurgery and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, USA

    Vladimir V. Didenko

Bibliographic Information

  • Book Title: Fast Detection of DNA Damage

  • Book Subtitle: Methods and Protocols

  • Editors: Vladimir V. Didenko

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7187-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7185-5Published: 14 July 2017

  • Softcover ISBN: 978-1-4939-8411-4Published: 11 June 2018

  • eBook ISBN: 978-1-4939-7187-9Published: 13 July 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 216

  • Number of Illustrations: 18 b/w illustrations, 24 illustrations in colour

  • Topics: Human Genetics

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