© 2017

Site-Specific Recombinases

Methods and Protocols

  • Nikolai¬†Eroshenko


  • Includes cutting-edge methods and protocols.

  • Provides step-by-step detail for reproducible results.

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1642)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Colin E. J. Pritchard, Lona J. Kroese, Ivo J. Huijbers
    Pages 21-35
  3. Yuanwu Ma, Lianfeng Zhang, Xingxu Huang
    Pages 37-52
  4. Eugenia Voziyanova, Rachelle P. Anderson, Yuri Voziyanov
    Pages 53-67
  5. Alfonso P. Farruggio, Mital S. Bhakta, Michele P. Calos
    Pages 69-85
  6. Avinash Chekuru, Veronika Kuscha, Stefan Hans, Michael Brand
    Pages 87-97
  7. Wei-Ming Chien, Yonggang Liu, Ana A. Dinca, Michael T. Chin
    Pages 99-107
  8. Jonathan C. Y. Tang, Stephanie Rudolph, Constance L. Cepko
    Pages 109-126
  9. Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson et al.
    Pages 127-150
  10. Susana Martin-Ortigosa, Brian G. Trewyn, Kan Wang
    Pages 169-180
  11. Bonnie M. Weasner, Jinjin Zhu, Justin P. Kumar
    Pages 195-209
  12. Douglas H. Roossien, Dawen Cai
    Pages 211-228
  13. Femi J. Olorunniji, Susan J. Rosser, W. Marshall Stark
    Pages 229-245
  14. Ze Peng, Nandita Nath, Zhiying Zhao, Jeff L. Froula, Jan-Fang Cheng, Feng Chen
    Pages 247-261
  15. Ze Peng, Jeff L. Froula, Jan-Fang Cheng
    Pages 263-284
  16. Femi J. Olorunniji, Christine Merrick, Susan J. Rosser, Margaret C. M. Smith, W. Marshall Stark, Sean D. Colloms
    Pages 303-323

About this book


This books aims to provide an up-to-date guide to using recombinases in the laboratory.  Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.


CRISPR protein design recombinase delivery recombinase specificity alteration synthetic biology

Editors and affiliations

  • Nikolai¬†Eroshenko
    • 1
  1. 1.Department of GeneticsHarvard Medical SchoolBostonUSA

Bibliographic information

  • Book Title Site-Specific Recombinases
  • Book Subtitle Methods and Protocols
  • Editors Nikolai Eroshenko
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-7167-1
  • Softcover ISBN 978-1-4939-8405-3
  • eBook ISBN 978-1-4939-7169-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 342
  • Number of Illustrations 25 b/w illustrations, 25 illustrations in colour
  • Topics Human Genetics
  • Buy this book on publisher's site