Book Volume 1642 2017

Site-Specific Recombinases

Methods and Protocols

Editors:

ISBN: 978-1-4939-7167-1 (Print) 978-1-4939-7169-5 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Protocol

    Pages 1-19

    Generating Genetically Modified Mice: A Decision Guide

  3. No Access

    Protocol

    Pages 21-35

    Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes

  4. No Access

    Protocol

    Pages 37-52

    Building Cre Knockin Rat Lines Using CRISPR/Cas9

  5. No Access

    Protocol

    Pages 53-67

    Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases

  6. No Access

    Protocol

    Pages 69-85

    Use of the DICE (Dual Integrase Cassette Exchange) System

  7. No Access

    Protocol

    Pages 87-97

    Ligand-Controlled Site-Specific Recombination in Zebrafish

  8. No Access

    Protocol

    Pages 99-107

    Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre

  9. No Access

    Protocol

    Pages 109-126

    Viral Delivery of GFP-Dependent Recombinases to the Mouse Brain

  10. No Access

    Protocol

    Pages 127-150

    Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors

  11. No Access

    Protocol

    Pages 151-168

    Marker Removal in Transgenic Plants Using Cre Recombinase Delivered with Potato Virus X

  12. No Access

    Protocol

    Pages 169-180

    Nanoparticle-Mediated Recombinase Delivery into Maize

  13. No Access

    Protocol

    Pages 181-194

    Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines

  14. No Access

    Protocol

    Pages 195-209

    FLPing Genes On and Off in Drosophila

  15. No Access

    Protocol

    Pages 211-228

    Imaging Neural Architecture in Brainbow Samples

  16. No Access

    Protocol

    Pages 229-245

    Purification and In Vitro Characterization of Zinc Finger Recombinases

  17. No Access

    Protocol

    Pages 247-261

    Preparing Mate-Paired Illumina Libraries Using Cre Recombinase

  18. No Access

    Protocol

    Pages 263-284

    Preparing Fosmid Mate-Paired Libraries Using Cre-LoxP Recombination

  19. No Access

    Protocol

    Pages 285-302

    Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways

  20. No Access

    Protocol

    Pages 303-323

    Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family

  21. No Access

    Protocol

    Pages 325-339

    Production of Minicircle DNA Vectors Using Site-Specific Recombinases

  22. Back Matter

    Pages 341-342