© 2017

Genome Editing in Animals

Methods and Protocols

  • Izuho Hatada


  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1630)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Hiroshi Ochiai, Takashi Yamamoto
    Pages 1-24
  3. Tetsushi Sakuma, Takashi Yamamoto
    Pages 25-36
  4. Izuho Hatada, Takuro Horii
    Pages 37-42
  5. Takuro Horii, Izuho Hatada
    Pages 55-66
  6. Takehito Kaneko
    Pages 101-108
  7. Masahito Watanabe, Hiroshi Nagashima
    Pages 121-139
  8. Zhen Liu, Yijun Cai, Qiang Sun
    Pages 141-152
  9. Jae Yong Han, Hong Jo Lee
    Pages 153-163
  10. Satoshi Ansai, Masato Kinoshita
    Pages 175-188
  11. Takuya Tsubota, Hideki Sezutsu
    Pages 205-218
  12. Takahito Watanabe, Sumihare Noji, Taro Mito
    Pages 219-233
  13. Yasunori Sasakura, Keita Yoshida, Nicholas Treen
    Pages 235-245

About this book


This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.


enzymes TALEN ZFN CRISPR zebra fish

Editors and affiliations

  • Izuho Hatada
    • 1
  1. 1.Laboratory of Genome Science Biosignal Genome Resource Center Institute for Molecular and Cellular RegulationGunma UniversityMaebashiJapan

Bibliographic information

  • Book Title Genome Editing in Animals
  • Book Subtitle Methods and Protocols
  • Editors Izuho Hatada
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-7127-5
  • Softcover ISBN 978-1-4939-8393-3
  • eBook ISBN 978-1-4939-7128-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 256
  • Number of Illustrations 22 b/w illustrations, 45 illustrations in colour
  • Topics Human Genetics
  • Buy this book on publisher's site