Ebolaviruses

1. Studying Infectious Ebolaviruses In Vitro

   1. Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence

      • Emily V. Nelson, Kristina M. Schmidt

      Pages 211-225

   2. Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells

      • Judith Olejnik, Emily V. Nelson

      Pages 227-241

   3. Electron Microscopy of Ebola Virus-Infected Cells

      • Takeshi Noda

      Pages 243-250

   4. Validating the Inactivation Effectiveness of Chemicals on Ebola Virus

      • Elaine Haddock, Friederike Feldmann

      Pages 251-257

   5. Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS)

      • Erik P. Carter, Elif Ç. Seymour, Steven M. Scherr, George G. Daaboul, David S. Freedman, M. Selim Ünlü et al.

      Pages 259-270

2. Studying Infectious Ebolaviruses In Vivo

   1. Front Matter

      Pages 271-271

      PDF

   2. Assessing Antiviral Countermeasures Using Mouse Models of Ebolavirus Infection

      • Andrea Kroeker, Bryan D. Griffin, Xiangguo Qiu, Gary Kobinger

      Pages 273-282

   3. Evaluation of Ebola Virus Countermeasures in Guinea Pigs

      • Andrea Marzi

      Pages 283-291

   4. Evaluation of Medical Countermeasures Against Ebolaviruses in Nonhuman Primate Models

      • Chad E. Mire, Thomas W. Geisbert

      Pages 293-307

   5. Quantification of Filovirus Glycoprotein-Specific Antibodies

      • Wakako Furuyama, Hiroko Miyamoto, Reiko Yoshida, Ayato Takada

      Pages 309-320

   6. Monitoring Innate Immune Gene Responses in the Hamster Model of Ebola Virus Disease by RT-PCR

      • Marko Zivcec

      Pages 321-337

3. Ebolaviruses in the Field

   1. Front Matter

      Pages 339-339

      PDF

   2. Real-Time and End-Point PCR Diagnostics for Ebola Virus

      • Allen Grolla

      Pages 341-352

   3. Production of Antigens for ELISA

      • Robert W. Cross, Thomas G. Ksiazek

      Pages 353-362

   4. ELISA Methods for the Detection of Ebolavirus Infection

      • Robert W. Cross, Thomas G. Ksiazek

      Pages 363-372

   5. Ebola Virus Field Sample Collection

      • Brian R. Amman, Amy J. Schuh, Jonathan S. Towner

      Pages 373-393

4. Back Matter

   Pages 395-397

   PDF

This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ebolaviruses: Methods and Protocols serves as a guide to the next generations of filovirus researchers and also helps to bring experts from other areas into the filovirus research arena.

• Ebola virus
• Virology
• Zaire ebolavirus
• Biosafety levels
• Filoviruses
• Immune response
• infectious diseases

“This book comprises three review chapters on filoviruses and 27 protocol chapters. … Scientists working on filoviruses and those wishing to enter the field will find a compendium of carefully written methods and protocols relevant to their work. … What makes the book stand out from many other volumes in this series is the inclusion of the three review chapters, which by themselves earn the book a spot on the bookshelf of any scientist working in the field.” (Christoph Wirblich, Doody's Book Reviews, January, 2018)

• Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany

  Thomas Hoenen, Allison Groseth

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