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RNAi and Small Regulatory RNAs in Stem Cells

Methods and Protocols

  • Baohong Zhang

Part of the Methods in Molecular Biology book series (MIMB, volume 1622)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

  3. RNA Interference

    1. Front Matter
      Pages 27-27
    2. Roman Galeev, Christine Karlsson, Aurélie Baudet, Jonas Larsson
      Pages 29-50
    3. Jiao Wang, Qian Wang, Fangfang Zhou, Dong Wang, Tieqiao Wen
      Pages 51-61
    4. Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, Mario Löhr
      Pages 63-79
    5. Soizik Berlivet, Isabelle Hmitou, Hélène Picaud, Matthieu Gérard
      Pages 91-100
    6. Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, Yumei Fu
      Pages 131-138
    7. Mario Löhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, György A. Homola et al.
      Pages 149-159
    8. Amelia J. Blake, Danielle S. Finger, Victoria L. Hardy, Elizabeth T. Ables
      Pages 161-184
  4. MicroRNAs

  5. CRISPR-Cas9 and Genome Editing

    1. Front Matter
      Pages 267-267
    2. Yinghua Wang, Yifu Ding, Jinsong Li
      Pages 293-305
  6. Back Matter
    Pages 307-311

About this book

Introduction

This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functional analysis, as well as usage of CRISPR/Cas 9 to knockout an individual gene for functional studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature.

Keywords

Stem cell differentiation MicroRNAs RNA interference CRISPR-Cas9 Regenerative medicine Gene knockdown

Editors and affiliations

  • Baohong Zhang
    • 1
  1. 1.Department of BiologyEast Carolina UniversityGreenvilleUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7108-4
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7106-0
  • Online ISBN 978-1-4939-7108-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site