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Serum/Plasma Proteomics

Methods and Protocols


(view affiliations)
  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1619)

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USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7057-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 149.99
Price excludes VAT (USA)
Hardcover Book
USD 219.99
Price excludes VAT (USA)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xvi
  2. Updated Blood Processing and Handling Strategies

    1. Front Matter

      Pages 1-1
    2. Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation

      • Viviana Greco, Cristian Piras, Luisa Pieroni, Andrea Urbani
      Pages 3-21
    3. A Protocol for the Preparation of Cryoprecipitate and Cryo-depleted Plasma for Proteomic Studies

      • Rosemary L. Sparrow, Richard J. Simpson, David W. Greening
      Pages 23-30
    4. Preparation of Platelet Concentrates for Research and Transfusion Purposes

      • David W. Greening, Richard J. Simpson, Rosemary L. Sparrow
      Pages 31-42
  3. Updated Fractionation Strategies for In-depth Blood Proteome Analysis

    1. Front Matter

      Pages 43-43
    2. Bead-Based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture

      • Elin Birgersson, Jochen M. Schwenk, Burcu Ayoglu
      Pages 45-54
    3. Affinity Proteomics for Fast, Sensitive, Quantitative Analysis of Proteins in Plasma

      • John P. O’Grady, Kevin W. Meyer, Derrick N. Poe
      Pages 55-61
    4. Characterization of the Low-Molecular-Weight Human Plasma Peptidome

      • David W. Greening, Richard J. Simpson
      Pages 63-79
    5. In-Depth, Reproducible Analysis of Human Plasma Using IgY 14 and SuperMix Immunodepletion

      • Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, David W. Speicher
      Pages 81-101
    6. Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometry

      • Dong Huey Cheon, Eun Gyeong Yang, Cheolju Lee, Ji Eun Lee
      Pages 103-117
    7. Identification of Core-Fucosylated Glycoproteome in Human Plasma

      • Qichen Cao, Qing Zhao, Xiaohong Qian, Wantao Ying
      Pages 127-137
  4. Updated Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids

    1. Front Matter

      Pages 139-139
    2. Proteomic Analysis of Blood Extracellular Vesicles in Cardiovascular Disease by LC-MS/MS Analysis

      • Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M. Ruilope, Maria G. Barderas
      Pages 141-149
    3. Targeted Approach for Proteomic Analysis of a Hidden Membrane Protein

      • Tania Martins-Marques, Sandra I. Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, Henrique Girao
      Pages 151-172
    4. Red Blood Cells in Clinical Proteomics

      • Ana Sofia Carvalho, Manuel S. Rodriguez, Rune Matthiesen
      Pages 173-181
    5. High-Throughput Quantitative Lipidomics Analysis of Nonesterified Fatty Acids in Plasma by LC-MS

      • Nicolas Christinat, Delphine Morin-Rivron, Mojgan Masoodi
      Pages 183-191
    6. Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry

      • Susanne Sales, Oskar Knittelfelder, Andrej Shevchenko
      Pages 203-212

About this book

Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Protocols, Second Edition is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays,  proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids.


  • Human blood
  • serum components
  • blood proteomics
  • post-translational modifications
  • blood proteome analysis
  • blood cell components

Editors and Affiliations

  • Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia

    David W. Greening, Richard J. Simpson

Bibliographic Information

  • Book Title: Serum/Plasma Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: David W. Greening, Richard J. Simpson

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-7056-8

  • Softcover ISBN: 978-1-4939-8380-3

  • eBook ISBN: 978-1-4939-7057-5

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 551

  • Number of Illustrations: 24 b/w illustrations, 70 illustrations in colour

  • Topics: Protein Biochemistry

Buying options

USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-7057-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 149.99
Price excludes VAT (USA)
Hardcover Book
USD 219.99
Price excludes VAT (USA)