Bacterial Protein Secretion Systems

Methods and Protocols

  • Laure Journet
  • Eric Cascales

Part of the Methods in Molecular Biology book series (MIMB, volume 1615)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Henrik Nielsen
    Pages 23-57
  3. Melissa Petiti, Laetitia Houot, Denis Duché
    Pages 59-64
  4. Maria Guillermina Casabona, Mylène Robert-Genthon, Didier Grunwald, Ina Attrée
    Pages 65-74
  5. Rhys A. Dunstan, Iain D. Hay, Trevor Lithgow
    Pages 81-86
  6. Anna Konovalova
    Pages 87-95
  7. Maxence S. Vincent, Eric Cascales
    Pages 97-103
  8. Gouzel Karimova, Daniel Ladant
    Pages 129-142
  9. Gang Li, S. Peter Howard
    Pages 143-149
  10. Yoann G. Santin, Eric Cascales
    Pages 151-158
  11. Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, Daniel Ladant
    Pages 159-176
  12. Jer-Sheng Lin, Erh-Min Lai
    Pages 177-187
  13. Krishnamohan Atmakuri
    Pages 189-197
  14. Laureen Logger, Abdelrahim Zoued, Eric Cascales
    Pages 199-210
  15. Jer-Sheng Lin, Erh-Min Lai
    Pages 211-219
  16. Julie P. M. Viala, Emmanuelle Bouveret
    Pages 221-232

About this book

Introduction

This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.

Keywords

Fractionation Lipoproteins Peptidoglycan Protease sub-cellular localisation

Editors and affiliations

  • Laure Journet
    • 1
  • Eric Cascales
    • 2
  1. 1.Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRSMarseilleFrance
  2. 2.Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRSMarseilleFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7033-9
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7031-5
  • Online ISBN 978-1-4939-7033-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book