© 2017

Poly(ADP-Ribose) Polymerase

Methods and Protocols

  • Alexei V. Tulin
  • Includes updated, cutting-edge methods and protocols for the study of PARP research

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1608)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Detection and Quantification of pADPr

    1. Front Matter
      Pages 1-1
    2. Tabea Zubel, Rita Martello, Alexander Bürkle, Aswin Mangerich
      Pages 3-18
    3. Yi-Chen Lai, Rajesh K. Aneja, Margaret A. Satchell, Robert S. B. Clark
      Pages 27-43
    4. Magali R. VanLinden, Marc Niere, Andrey A. Nikiforov, Mathias Ziegler, Christian Dölle
      Pages 45-56
  3. Identification of Protein Targets

    1. Front Matter
      Pages 69-69
    2. Rory K. Morgan, Michael S. Cohen
      Pages 71-77
    3. Casey M. Daniels, Shao-En Ong, Anthony K. L. Leung
      Pages 79-93
    4. Sara C. Larsen, Mario Leutert, Vera Bilan, Rita Martello, Stephanie Jungmichel, Clifford Young et al.
      Pages 149-162
  4. Functional Analysis

    1. Front Matter
      Pages 163-163
    2. Théo Lebeaupin, Rebecca Smith, Sébastien Huet, Gyula Timinszky
      Pages 165-183
    3. Edit Mikó, Tünde Kovács, Tamás Fodor, Péter Bai
      Pages 185-200
    4. Manana Melikishvili, Elena Matveeva, Yvonne Fondufe-Mittendorf
      Pages 211-228
  5. Regulation of PARP-1 and Its Partners

    1. Front Matter
      Pages 229-229

About this book


This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr production during the cell cycle, the volume continues with sections on the identification of pADPr protein acceptors, methods focusing on studying molecular mechanisms of PARP functions in eukaryotic cells, particularly those involved in control of DNA repair and oxidative stress, as well as in expression regulation, approaches to the in vitro reconstitution of PARP-1 interaction with chromatin, the development and testing of small molecule PARP inhibitors, and the functions of understudied members of PARP family.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Second Edition serves as an ideal companion to the first edition for scientists whose investigations involve this important pathway.

The chapter 'Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach' is published open access under a CC BY 4.0 license.


PARP-1 pADPr Enzymatic activity DNA repair Protein acceptors PARP inhibitors

Editors and affiliations

  • Alexei V. Tulin
    • 1
  1. 1.Cancer Biology ProgramFox Chase Cancer CenterPhiladelphiaUSA

Bibliographic information

  • Book Title Poly(ADP-Ribose) Polymerase
  • Book Subtitle Methods and Protocols
  • Editors Alexei V. Tulin
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-6992-0
  • Softcover ISBN 978-1-4939-8361-2
  • eBook ISBN 978-1-4939-6993-7
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XV, 528
  • Number of Illustrations 35 b/w illustrations, 47 illustrations in colour
  • Topics Protein Science
  • Buy this book on publisher's site