Zygotic Genome Activation

Methods and Protocols

  • Kiho Lee

Part of the Methods in Molecular Biology book series (MIMB, volume 1605)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Boyang Liu, Jörg Grosshans
    Pages 11-30
  3. Malavika K. Adur, Benjamin J. Hale, Jason W. Ross
    Pages 63-81
  4. Noémie Ranisavljevic, Ikuhiro Okamoto, Edith Heard, Katia Ancelin
    Pages 133-145
  5. Ka Yi Ling, Lih Feng Cheow, Stephen R. Quake, William F. Burkholder, Daniel M. Messerschmidt
    Pages 171-189
  6. Lu Zhang, Zoltan Machaty
    Pages 207-217
  7. Veronika Benesova, Veronika Kinterova, Jiri Kanka, Tereza Toralova
    Pages 245-257
  8. Keisuke Aoshima, Takashi Kimura, Yuki Okada
    Pages 259-270
  9. Back Matter
    Pages 271-272

About this book

Introduction

This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA.

Keywords

ZGA Zygote embryogenesis epigenetics genetic engineering CRISPR

Editors and affiliations

  • Kiho Lee
    • 1
  1. 1.Department of Animal and Poultry SciencesVirginia TechBlacksburgUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6988-3
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6986-9
  • Online ISBN 978-1-4939-6988-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book