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  • © 2018

Hemorrhagic Fever Viruses

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1604)

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eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6981-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.99
Price excludes VAT (USA)
Hardcover Book USD 179.99
Price excludes VAT (USA)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvi
  2. Surveillance, Diagnosis and Classification of Hemorrhagic Fever Viruses

    1. Front Matter

      Pages 1-1
    2. Global Spread of Hemorrhagic Fever Viruses: Predicting Pandemics

      • Jean-Paul Gonzalez, Marc Souris, Willy Valdivia-Granda
      Pages 3-31Open Access
    3. An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses

      • Francisco J. Díaz, Luis E. Paternina, Juan David Rodas
      Pages 33-41
    4. Diagnostics for Lassa Fever: Detecting Host Antibody Responses

      • Maria S. Salvato, Igor S. Lukashevich, Sandra Medina-Moreno, Juan Carlos Zapata
      Pages 79-88
    5. Sampling Design and Mosquito Trapping for Surveillance of Arboviral Activity

      • Luís E. Paternina, Juan David Rodas
      Pages 89-100
    6. Epidemiological Surveillance of Rodent-Borne Viruses (Roboviruses)

      • Juan David Rodas, Andrés F. Londoño, Sergio Solari
      Pages 101-109
  3. Structural Studies and Reverse Genetics of Hemorrhagic Fever Viruses

    1. Front Matter

      Pages 111-111
    2. Entry Studies of New World Arenaviruses

      • María Guadalupe Martínez, María Belén Forlenza, Nélida A. Candurra, Sandra M. Cordo
      Pages 113-133
    3. Studies of Lassa Virus Cell Entry

      • Antonella Pasquato, Antonio Herrador Fernandez, Stefan Kunz
      Pages 135-155
    4. Assays to Assess Arenaviral Glycoprotein Function

      • Junjie Shao, Xiaoying Liu, Yuying Liang, Hinh Ly
      Pages 169-178
    5. Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus

      • Xiaoxuan Qi, Wenjian Wang, Haohao Dong, Yuying Liang, Changjiang Dong, Hinh Ly
      Pages 179-188
    6. Intracellular Detection of Viral Transcription and Replication Using RNA FISH

      • Michael E. Lindquist, Connie S. Schmaljohn
      Pages 201-207
    7. Hemorrhagic Fever Virus Budding Studies

      • Ronald N. Harty
      Pages 209-215

About this book

This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection.

Keywords

  • HFV
  • Zoonotic pathogens
  • Vaccine development
  • Phenotype identification
  • Pathogenesis

Editors and Affiliations

  • School of Medicine, University of Maryland, Baltimore, USA

    Maria S. Salvato

Bibliographic Information

  • Book Title: Hemorrhagic Fever Viruses

  • Book Subtitle: Methods and Protocols

  • Editors: Maria S. Salvato

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6981-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-6980-7

  • Softcover ISBN: 978-1-4939-8358-2

  • eBook ISBN: 978-1-4939-6981-4

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 425

  • Number of Illustrations: 9 b/w illustrations, 52 illustrations in colour

  • Topics: Immunology

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6981-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.99
Price excludes VAT (USA)
Hardcover Book USD 179.99
Price excludes VAT (USA)