ATM Kinase

Methods and Protocols

  • Sergei V. Kozlov

Part of the Methods in Molecular Biology book series (MIMB, volume 1599)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Hailiang Hu, Shareef Nahas, Richard A. Gatti
    Pages 1-11
  3. Guillaume Rieunier, Catherine Dubois D’Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, Marc-Henri Stern
    Pages 25-42
  4. Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, Andrew Plater
    Pages 43-56
  5. Shyam Nyati, Grant Young, Brian Dale Ross, Alnawaz Rehemtulla
    Pages 97-111
  6. Mark E. Graham, Martin F. Lavin, Sergei V. Kozlov
    Pages 127-144
  7. Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, Ryo Otomo
    Pages 145-156
  8. Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, Zbigniew Darzynkiewicz
    Pages 183-196
  9. Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, Amanda G. Paulovich
    Pages 197-213
  10. Marina E. Borisova, Sebastian A. Wagner, Petra Beli
    Pages 215-227
  11. Rossana Piccinno, Marta Cipinska, Vassilis Roukos
    Pages 263-275

About this book

Introduction

Ataxia–telangiectasia (A-T) is a rare and severe genetic disorder affecting children. A-T is a multisystem disease characterized by progressive neurodegeneration, immunodeficiency and cancer predisposition. This detailed volume explores the ever expanding field of research into the ATM (ataxia-telangiectasia, mutated) gene and the role played by ATM kinase in DNA damage signaling and diverse cellular processes. What follows is a handy desktop reference for both seasoned A-T researchers and postgraduate students, as it demonstrates the breadth of recent developments in A-T studies. Written for the highly successfulMethods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Both classic and cutting-edge techniques are described, including ATM gene mutation detection, assays for radiosensitivity and radioresistant DNA synthesis, new methods to measure ATM kinase activity by imaging microscopy and high content screening as well as proteomics, phosphoproteomics and bioinformatics approaches to decipher ATM-dependent signalling pathways. Additional methods include generation of patient-specific stem cells and approaches to study ATM functions in the nervous system. 

Comprehensive and practical, ATM Kinase: Methods and Protocols aims to ignite and attract the interest of colleagues from diverse fields to A-T research in an effort to bring their expertise and fresh ideas to resolve many A-T puzzles still waiting to be pieced together and to alleviate the suffering of A-T children and their families.

Keywords

Ataxia–telangiectasia (A-T) Genetic disorder DNA damage Mutated ATM gene

Editors and affiliations

  • Sergei V. Kozlov
    • 1
  1. 1.University of Queensland Centre for Clinical Research (UQCCR)University of QueenslandHerstonAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6955-5
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6953-1
  • Online ISBN 978-1-4939-6955-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book