Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Table of contents (18 protocols)
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Front Matter
Pages i-xiii
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- Taisuke Kadoshima, Hideya Sakaguchi, Mototsugu Eiraku
Pages 1-16
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- Atsushi Kuwahara, Tokushige Nakano, Mototsugu Eiraku
Pages 17-29
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- Keisuke Ishihara, Adrian Ranga, Matthias P. Lutolf, Elly M. Tanaka, Andrea Meinhardt
Pages 43-55
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- Chikafumi Ozone, Hidetaka Suga
Pages 57-65
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- Jing Nie, Karl R. Koehler, Eri Hashino
Pages 67-83
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- Francesco Antonica, Dominika Figini Kasprzyk, Andrea Alex Schiavo, Mírian Romitti, Sabine Costagliola
Pages 85-95
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- Masamitsu Oshima, Miho Ogawa, Takashi Tsuji
Pages 97-116
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- Kyosuke Asakawa, Koh-ei Toyoshima, Takashi Tsuji
Pages 117-134
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- Miho Ogawa, Takashi Tsuji
Pages 135-151
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- Masatoshi Hirayama, Kazuo Tsubota, Takashi Tsuji
Pages 153-165
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- Jorge O. Múnera, James M. Wells
Pages 167-177
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- Yasuhiro Yoshimura, Atsuhiro Taguchi, Ryuichi Nishinakamura
Pages 179-193
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- Minoru Takasato, Melissa H. Little
Pages 195-206
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- Keisuke Sekine, Takanori Takebe, Hideki Taniguchi
Pages 207-216
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- Taka-aki K. Noguchi, Akira Kurisaki
Pages 217-228
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- Maxime M. Mahe, Nicole E. Brown, Holly M. Poling, Michael A. Helmrath
Pages 229-245
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- Maxime M. Mahe, Nicole E. Brown, Holly M. Poling, Michael A. Helmrath
Pages E1-E1
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Back Matter
Pages 247-248
About this book
This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy. This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D cell culture and manipulation. The chapters cover topics such as generation of a 3D retinal tissue formation; functional salivary gland regeneration; liver regeneration using cultures liver bud; and in vivo model of small intestine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Organ Regeneration: 3D Stem Cell Culture and Manipulation is a valuable resource for scientists and researchers who are interested in this field.
Keywords
- Cerebellar
- Stem Cell
- Transplantation
- embryogenesis
- tissue replacement
Editors and Affiliations
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Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Japan
Takashi Tsuji