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  • © 2017

Organ Regeneration

3D Stem Cell Culture & Manipulation

Editors:

(view affiliations)
  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1597)

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eBook
USD 129.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6949-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 169.99
Price excludes VAT (USA)
Hardcover Book
USD 169.99
Price excludes VAT (USA)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
  2. Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture

    • Atsushi Kuwahara, Tokushige Nakano, Mototsugu Eiraku
    Pages 17-29
  3. Reconstitution of a Patterned Neural Tube from Single Mouse Embryonic Stem Cells

    • Keisuke Ishihara, Adrian Ranga, Matthias P. Lutolf, Elly M. Tanaka, Andrea Meinhardt
    Pages 43-55
  4. Functional Pituitary Tissue Formation

    • Chikafumi Ozone, Hidetaka Suga
    Pages 57-65
  5. Generation of Functional Thyroid Tissue Using 3D-Based Culture of Embryonic Stem Cells

    • Francesco Antonica, Dominika Figini Kasprzyk, Andrea Alex Schiavo, MĂ­rian Romitti, Sabine Costagliola
    Pages 85-95
  6. Functional Tooth Regeneration

    • Masamitsu Oshima, Miho Ogawa, Takashi Tsuji
    Pages 97-116
  7. Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells

    • Kyosuke Asakawa, Koh-ei Toyoshima, Takashi Tsuji
    Pages 117-134
  8. Functional Salivary Gland Regeneration

    • Miho Ogawa, Takashi Tsuji
    Pages 135-151
  9. Generation of a Bioengineered Lacrimal Gland by Using the Organ Germ Method

    • Masatoshi Hirayama, Kazuo Tsubota, Takashi Tsuji
    Pages 153-165
  10. Generation of Gastrointestinal Organoids from Human Pluripotent Stem Cells

    • Jorge O. MĂșnera, James M. Wells
    Pages 167-177
  11. Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells

    • Yasuhiro Yoshimura, Atsuhiro Taguchi, Ryuichi Nishinakamura
    Pages 179-193
  12. Liver Regeneration Using Cultured Liver Bud

    • Keisuke Sekine, Takanori Takebe, Hideki Taniguchi
    Pages 207-216
  13. In Vivo Model of Small Intestine

    • Maxime M. Mahe, Nicole E. Brown, Holly M. Poling, Michael A. Helmrath
    Pages 229-245
  14. Erratum to: In Vivo Model of Small Intestine

    • Maxime M. Mahe, Nicole E. Brown, Holly M. Poling, Michael A. Helmrath
    Pages E1-E1
  15. Back Matter

    Pages 247-248

About this book

This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy. This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D cell culture and manipulation. The chapters cover topics such as generation of a 3D retinal tissue formation; functional salivary gland regeneration; liver regeneration using cultures liver bud; and in vivo model of small intestine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Organ Regeneration: 3D Stem Cell Culture and Manipulation is a valuable resource for scientists and researchers who are interested in this field.

Keywords

  • Cerebellar
  • Stem Cell
  • Transplantation
  • embryogenesis
  • tissue replacement

Editors and Affiliations

  • Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Japan

    Takashi Tsuji

Bibliographic Information

  • Book Title: Organ Regeneration

  • Book Subtitle: 3D Stem Cell Culture & Manipulation

  • Editors: Takashi Tsuji

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6949-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6947-0

  • Softcover ISBN: 978-1-4939-8348-3

  • eBook ISBN: 978-1-4939-6949-4

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 248

  • Number of Illustrations: 7 b/w illustrations, 60 illustrations in colour

  • Topics: Cell Biology

Buying options

eBook
USD 129.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6949-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 169.99
Price excludes VAT (USA)
Hardcover Book
USD 169.99
Price excludes VAT (USA)